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KIAA0232 Antibody | 1258

KIAA0232 Antibody | 1258 gallery image 1

Western blot analysis of mouse brain tissue extract probed with HuCal anti KIA000232 (AbyD01258) followed by goat anti human IgG F(ab’)2:HRP (0500-0099)

KIAA0232 Antibody | 1258 gallery image 2

Western blot of KIAA0232 in a range of mouse tissues using HCA028. Endogenous protein is detected only in brain tissue, and not in any other sample tested

  • KIAA0232 Antibody | 1258 thumbnail image 1
  • KIAA0232 Antibody | 1258 thumbnail image 2
  • Human anti Mouse KIAA0232
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA028E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • HCA028 recognises a mouse protein previously only described as the hypothetical protein KIAA0232.

      This antibody has been used to demonstrate expression of KIAA0232 protein in murine brain, and studies have not detected expression in any other tissue tested, including heart, lung, liver, muscle, kidney, testis, pancreas, thymus, spleen and prostate.
    • Intended Use
    • Target Species
    • Product Form
      A bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the anti-body heavy chain.
    • Reconstitution
      Reconstitute with 0.1ml distilled water
    • Preparation
      Metal chelate affinity chromatography
    • Preservative Stabilisers
      None present.
    • Immunogen
      Recombinant 127aa polypeptide corresponding to nt 3750-4130 of the cDNA
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 1mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
    • Shelf Life
      6 months from date of reconstitution.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1001/500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional KIAA0232 Antibody Formats

    Formats Clone Applications Sizes available
    KIAA0232 Antibody : Purified 1258 E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Mouse anti c-Myc:HRPMCA2200P0.1 mgC, E, P, WB*
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • KIAA0232 Antibody | 1258 thumbnail image 1
                • KIAA0232 Antibody | 1258 thumbnail image 2


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