Kappa Light Chain Antibody | OX-20

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Kappa Light Chain Antibody | OX-20 gallery image 1

A Mouse monoclonal antibody of the IgG1 isotype was run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1 and 5. 1, 0.5 and 0.25 ug of antibody were run in lanes 2, 3 and 4 and 6, 7 and 8 respectively. Protein detection was carried out on lanes 2, 3 and 4 using Goat anti Mouse IgG (H/L):HRP (STAR207P) at a dilution of 1/10,000. Detection was carried out on lanes 6, 7 and 8 using Rat anti Mouse Kappa Light Chain:HRP (MCA152P) at a dilution of 1/1,000. Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure time. STAR207P was specific for both the heavy (50kDa) and light (25kDa) chains whereas MCA152P was specific for the light chains

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Kappa Light Chain Antibody | OX-20 gallery image 2

Purified Mouse IgG (PMP01) used as coating reagent in a direct ELISA detected with Rat anti Mouse Kappa Light Chain:HRP (MCA152P)

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Kappa Light Chain Antibody | OX-20 gallery image 3

HeLa (lanes 2, 5 and 8) and HepG2 (lanes 3, 6 and 9) cell lysates were run under reducing conditions on SDS PAGE using the Bio-Rad V3 system and transferred to a PVDF membrane. Bio-Rad Precision Plus molecular weight marker was run in lanes 1, 4 and 7. A Mouse anti Human GAPDH (MCA4740) was used as the primary antibody at a dilution of 1/1000. Rat anti Mouse Kappa Light Chain:HRP (MCA152P) was used as the secondary antibody at 1/1000 (lanes 2 and 3), 1/5000 (lanes 5 and 6) and 1/10000 (lanes 8 and 9). Visualization was carried out using the Bio-Rad ChemiDoc with automatic exposure

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  • Rat anti Mouse Kappa Light Chain:HRP
  • Rat anti Mouse Kappa Light Chain
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  • Product Type
    Monoclonal Antibody
  • Clone
    OX-20
  • Isotype
    IgG2b
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA152PE, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA152P
    MCA152Fdatasheet pdfdatasheet pdf1 mg
    MCA152
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse kappa light chain antibody, clone OX-20 recognizes the kappa light chain of murine immunoglobulins. OX-20 does not recognize mouse lambda light chains or bind to the immunoglobulin heavy chain.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      0.01% Thiomersal
      HRP Stabiliser (BUF052A)
      0.09%Sodium Azide
    • Immunogen
      Purified mouse IgG
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from an immunised PVG.RT1c rat were fused with cells of the rat Y3 Ag.1.2.3 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC for one month or at -20oC for longer.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/10,000
      Western Blotting1/10001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/100

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Kappa Light Chain Antibody Formats

    Formats Clone Applications Sizes available
    Kappa Light Chain Antibody : HRP OX-20 E, WB 0.1 mg
    Kappa Light Chain Antibody : Purified OX-20 F 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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