IL-6 Antibody

//www.bio-rad-antibodies.com/mouse-il-6-antibody-aam15g.html?RenderPageSpecific=1
IL-6 Antibody gallery image 1

Purchased this product? Why not be the first to submit a review and upload an image?

Enlarge
  • IL-6 Antibody thumbnail image 1
  • Rabbit anti Mouse Interleukin-6:Biotin
  • Rabbit anti Mouse Interleukin-6
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM15GE, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM15G
    AAM15BE, WB*datasheet pdfdatasheet pdf50 µg
    AAM15B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse Interleukin-6 antibody recognizes mouse Interleukin-6 (IL-6). Rabbit anti Mouse Interleukin-6 antibody neutralizes murine IL-6 activity with an ND50 for mIL-6 (3 pgml-1 of between 0.24 and 0.36μg ml-1.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      Reconstitute with 0.1ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
    • Antiserum Preparation
      Antisera to murine interleukin 6 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by antigen affinity chromatography.
    • Preservative Stabilisers
      None present
      None present
    • Immunogen
      Recombinant murine IL-6
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.1mg/ml after reconstitution.
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution
    • GO Terms
      external side of plasma membrane
      endocrine pancreas development
      positive regulation of transcription, DNA-dependent
      branching involved in salivary gland morphogenesis
      neutrophil apoptosis
      interleukin-6 receptor binding
      negative regulation of chemokine biosynthetic process
      growth factor activity
      defense response to protozoan
      positive regulation of T-helper 2 cell differentiation
      interleukin-6-mediated signaling pathway
      cytokine activity
      positive regulation of epithelial cell proliferation
      muscle cell homeostasis
      immune response
      acute-phase response
      negative regulation of hormone secretion
      extracellular space
      epithelial cell proliferation involved in salivary gland morphogenesis
      glucagon secretion
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.15ug/ml0.30ug/ml
      Western Blotting(1)0.1ug/ml0.2ug/ml
      (1)
      Reducing or non-reducing conditions.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.52.0ug/ml
      Functional Assays5.08.0ng/ml
      Western Blotting0.10.2ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
      This biotinylated mouse IL-6 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified mouse IL-6 antibody (AAM15G) as the capture reagent .
    • ELISA
      This purified mouse IL-6 antibody may be used in an indirect ELISA or as the capture reagent in a sandwich ELISA with a biotinylated mouse IL-6 antibody (AAM15B) as the detection reagent.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
      This purified mouse IL-6 antibody may used in Western Blotting applications under either reducing or non-reducing conditions.
    • Instructions For Use
    • Instructions For Use

    Additional IL-6 Antibody Formats

    Formats Applications Sizes available
    IL-6 Antibody : Purified E, FN, WB 0.1 mg
    IL-6 Antibody : Biotin E, WB* 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Write your review

              You may also be interested in...