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IL-4 gallery image 1

Published customer image:
Murine macrophages cultured in the presence of mouse interferon-γ (PMP43A ) or mouse interleukin-4 (PMP81) to generate an M1 and M2 phenotype respectively. cells were sublequently exposed to bacteria as indicated to visualise phagocytosis.
Image caption:
3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages.
(A,B,C) Maximum intensity projection and (D,E,F) the corresponding slice view 3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages (60:1 BMR, 1 hour exposure time), respectively, on the Deltavision OMX Structured Illumination Microscope V4 Blaze (Applied Precision, WA, USA). P. gingivalis W50 were labelled with AF488 (green) while the nuclei and actin filaments of the macrophages were stained with DAPI (blue) and Phalloidin-TRITC (red) respectively. The slice images were obtained from a section with defined size (between 10–50 z-stack) to confirm phagocytosis of P. gingivalis W50 by naive, M1 and M2 macrophages.

From: Lam RS, O'Brien-Simpson NM, Holden JA, Lenzo JC, Fong SB, Reynolds EC (2016)
Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS ONE 11(7): e0158629.

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  • Recombinant Mouse Interleukin-4
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    PMP81E, FN, WBdatasheet pdfdatasheet pdf20 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Recombinant Murine interleukin-4 is an E.coli derived preparation of Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1. Interleukin-4 is a pleiotropic cytokine which interacts with multiple cell lineages, including T cells, fibroblasts and haematopoietic cells, and is one of the most important factors involved in B cell IgG1 and IgE class switching. IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays
      Western Blotting1.53.0ng/lane

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
      PMP81 may be used as a standard in ELISA application involving either AAM36 or AAM36B.
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      PMP81 may be used as a positive control for western blotting applications involving either AAM36 or AAM36B.
    • Instructions For Use

    Additional IL-4 Formats

    Formats Applications Sizes available
    IL-4 : Rec. Protein E, FN, WB 20 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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