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Murine macrophages cultured in the presence of mouse interferon-γ (PMP43A ) or mouse interleukin-4 (PMP81) to generate an M1 and M2 phenotype respectively. cells were sublequently exposed to bacteria as indicated to visualise phagocytosis.
3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages.
(A,B,C) Maximum intensity projection and (D,E,F) the corresponding slice view 3D-SIM images of P. gingivalis W50 phagocytosed by naive, M1 and M2 macrophages (60:1 BMR, 1 hour exposure time), respectively, on the Deltavision OMX Structured Illumination Microscope V4 Blaze (Applied Precision, WA, USA). P. gingivalis W50 were labelled with AF488 (green) while the nuclei and actin filaments of the macrophages were stained with DAPI (blue) and Phalloidin-TRITC (red) respectively. The slice images were obtained from a section with defined size (between 10–50 z-stack) to confirm phagocytosis of P. gingivalis W50 by naive, M1 and M2 macrophages.
From: Lam RS, O'Brien-Simpson NM, Holden JA, Lenzo JC, Fong SB, Reynolds EC (2016)
Unprimed, M1 and M2 Macrophages Differentially Interact with Porphyromonas gingivalis.
PLoS ONE 11(7): e0158629.