IL-4 antibody | BVD4-1D11

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1ug/ml	5ug/ml
Flow Cytometry 1		1/10	1/50
Immunohistology - Frozen
Western Blotting		1ug/ml	5ug/ml

1. ¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry

Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

References for IL-4 antibody

1. Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
   Immunol Rev. 127: 5-24.
2. Sander, B. et al. (1993) Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining.
   J Immunol Methods. 166 (2): 201-14.
3. Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
   J Exp Med. 192 (6): 823-33.
4. Finkelman, F.D. et al. (2000) Stat6 regulation of in vivo IL-4 responses.
   J Immunol. 164: 2303-10.
5. Hochrein, H. et al. (2000) Interleukin (IL)-4 is a major regulatory cytokine governing bioactive IL-12 production by mouse and human dendritic cells.
   J Exp Med. 192: 823-33.
6. Urban, J.F. Jr. et al. (2000) Stat6 signaling promotes protective immunity against Trichinella spiralis through a mast cell- and T cell-dependent mechanism.
   J Immunol. 164: 2046-52.
7. Yang, Y.F. et al. (2000) Requirement for IFN-gamma in IL-12 production induced by collaboration between v(alpha)14(+) NKT cells and antigen-presenting cells.
   Int Immunol. 12: 1669-75.
8. Pechová, J. et al. (2004) Effect of tick salivary gland extract on the cytokine production by mouse epidermal cells.
   Folia Parasitol (Praha). 51: 367-72.
View The Latest Product References
9. Roy, E. et al. (2008) Beneficial effect of anti-interleukin-4 antibody when administered in a murine model of tuberculosis infection.
   Tuberculosis (Edinb). 88 (3): 197-202.

Functional Assays

RRID

AB_322398

UniProt

P07750

Entrez Gene

Il4

GO Terms

GO:0005125 cytokine activity

GO:0005136 interleukin-4 receptor binding

GO:0005615 extracellular space

GO:0006955 immune response

GO:0008083 growth factor activity

GO:0008203 cholesterol metabolic process

GO:0030890 positive regulation of B cell proliferation

GO:0042104 positive regulation of activated T cell proliferation

GO:0042113 B cell activation

View More GO Terms

GO:0042523 positive regulation of tyrosine phosphorylation of Stat5 protein

GO:0043066 negative regulation of apoptosis

GO:0043193 positive regulation of gene-specific transcription

GO:0045348 positive regulation of MHC class II biosynthetic process

GO:0045671 negative regulation of osteoclast differentiation

GO:0045944 positive regulation of transcription from RNA polymerase II promoter

GO:0048295 positive regulation of isotype switching to IgE isotypes

GO:0048304 positive regulation of isotype switching to IgG isotypes

GO:0051091 positive regulation of transcription factor activity

MCA1304G

151117 161041 164020 1804

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