PMP46 is an Escherichia coli-derived recombinant proteins corresponding to full-length mature mouse interleukin-13, a secreted cytokine involved in the regulation of inflammatory and immune responses.
Purified recombinant protein - lyophilised
Reconstitute with 10ul distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Recombinant protein expressed in E. coli
2.5 x 105 Units/mg
>98% by SDS PAGE and HPLC
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml after reconstitution.
Protein Molecular Weight
12.3kD (111 amino acid sequence)
Reagents In The Kit
Preparing The Antibody
10mM Sodium Citrate pH4.0
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.
PMP46 may be used as standard for ELISA applications involving AAM34 or AAM34B.
Histology Positive Control Tissue
PMP46 may be used as a positive control for western blotting applications involving AAM34 or AAM34B.