Recombinant mouse IL-12 is a 70.5kDa heterodimeric glycoprotein consisting of disulfide-linked p35 and p40 subunits. IL-12 is a potent regulator of cell-mediated immune responses. It induces production of interferon gamma in natural killer and T-cells.
Purified recombinant protein - lyophilised
Reconstitute with 0.1ml sterile PBS containing 0.1% bovine serum albumin Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Recombinant protein expressed in CHO cells.
1 x 107 units/mg
>98% by SDS PAGE + HPLC analysis
Approx. Protein Concentrations
0.1 mg/ml after reconstitution.
Protein Molecular Weight
75kD (35kDa and 40kDa subunits) - 506 amino acid residues
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate positive/negative controls.