IgG2c Antibody

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Published customer image:
Horseradish peroxidase conjugated Goat anti Mouse IgG2c antibody (STAR135P) used to evaluate IgG2c levels in mouse samples by ELISA.
Image caption:
rPmpD-specific IgG1 and IgG2c responses following immunization with different rPmpD vaccine formulations in C57BL/6 mice. Anti-rPmpD serum IgG1 and IgG2c titres were measured two weeks following penultimate and final immunisations (A, C) or six weeks post-challenge (B, D). Results are expressed as the geometric mean ± the standard deviation for groups of 5 mice measured in triplicate, and are representative of two separate experiments. (*P<0.05, **P<0.01).

From: Paes W, Brown N, Brzozowski AM, Coler R, Reed S, Carter D, Bland M, Kaye PM, Lacey CJ.
Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice. Vaccine. 2016 Jul 29;34(35):4123-31.

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  • IgG2c Antibody thumbnail image 1
  • Goat anti Mouse IgG2c:Alk. Phos.
  • Goat anti Mouse IgG2c:HRP
  • Goat anti Mouse IgG2c:FITC
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR135AEdatasheet pdfdatasheet pdf0.5 mg
    STAR135A
    STAR135PEdatasheet pdfdatasheet pdf0.5 mg
    STAR135P
    STAR135FFdatasheet pdfdatasheet pdf0.5 mg
    STAR135F
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse IgG2c antibody recognizes mouse IgG2c, otherwise known as the Igh 1b allele of IgG2a. Goat anti Mouse IgG2c antibody is assessed by ELISA and immunoelectrophoresis and has been shown to react with IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice and does not react with L-chains or other IgG subclasses.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Antiserum Preparation
      Antisera to mouse IgG2c were raised by repeated immunisations of goats with highly purified antigen.
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
      0.1%Pro-CleanTM400
      0.2%Bovine Serum Albumin
      0.09%Sodium Azide
      0.2%Bovine Serum Albumin
    • Immunogen
      Native mouse IgG2c.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      50mM HEPES, 0.1M Nacl, 1mM Mgcl2, 0.1mM Zncl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.
      DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/100001/100000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG2c Antibody Formats

    Formats Applications Sizes available
    IgG2c Antibody : FITC F 0.5 mg
    IgG2c Antibody : HRP E 0.5 mg
    IgG2c Antibody : Alk. Phos. E 0.5 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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