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Horseradish peroxidase conjugated Goat anti Mouse IgG2c antibody (STAR135P) used to evaluate IgG2c levels in mouse samples by ELISA.
rPmpD-specific IgG1 and IgG2c responses following immunization with different rPmpD vaccine formulations in C57BL/6 mice. Anti-rPmpD serum IgG1 and IgG2c titres were measured two weeks following penultimate and final immunisations (A, C) or six weeks post-challenge (B, D). Results are expressed as the geometric mean ± the standard deviation for groups of 5 mice measured in triplicate, and are representative of two separate experiments. (*P<0.05, **P<0.01).
From: Paes W, Brown N, Brzozowski AM, Coler R, Reed S, Carter D, Bland M, Kaye PM, Lacey CJ.
Recombinant polymorphic membrane protein D in combination with a novel, second-generation lipid adjuvant protects against intra-vaginal Chlamydia trachomatis infection in mice. Vaccine. 2016 Jul 29;34(35):4123-31.