IgG2a Antibody

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    108008Edatasheet pdfdatasheet pdf1 mg
    108008
    108007Fdatasheet pdfdatasheet pdf1 mg
    108007
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Goat anti Mouse IgG2a is specific to mouse immunoglobulin G, isotype 2a.

      This antibody has beed cross-adsorbed against mouse IgA, IgG1, IgG2b, IgG3, IgM as well as pooled human sera and human papraproteins.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanno
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      IgG conjugated to Biotin - liquid
      IgG conjugated to Texas Red® - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse IgG2a were raised by repeated immunisations of goats with highly purified antigen. Purified Ig was prepared from whole serum by affinity chromatography on mouse IgG2a covalently linked to agarose. This preparation has been cross absorbed with mouse IgM, IgG1, IgG2b, IgG3 and IgA to ensure class specificity.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Mouse IgG2a
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date
      Please see label for expiry date
    • GO Terms
      humoral immune response mediated by circulating immunoglobulin
      antigen binding
      integral to membrane
      immunoglobulin complex, circulating
      antibacterial humoral response
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Texas Red® is a registered trademark of Molecular Probes, Inc
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IgG2a Antibody Formats

    Formats Applications Sizes available
    IgG2a Antibody : Biotin E 1 mg
    IgG2a Antibody : Texas Red® F 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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