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IgG antibody

Rabbit F(ab')2 anti Mouse IgG:RPE

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
IgG

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STAR12A
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Rabbit F(ab')2 anti Mouse IgG antibody recognizes mouse IgG

Target Species
Mouse
Product Form
F(ab')2 fragment of IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Antiserum Preparation
Antisera to mouse IgG were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Mouse IgG.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul.

How to Use the Spectraviewer

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References for IgG antibody

  1. Dasgupta, G. et al. (2011) Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.
    Invest Ophthalmol Vis Sci. 52 (6): 3321-33.
  2. Jones, D.C. et al. (2011) HLA Class I Allelic Sequence and Conformation Regulate Leukocyte Ig-Like Receptor Binding.
    J Immunol. 186: 2990-7.

RRID
AB_321922
UniProt
P01869
P03987
P01867
P01864
P01865
P01868
P01863
Entrez Gene
Igh-1a
Igh-1a
Igh-1a
Ighg2b
Ighg1
Ighg1
AI324046
GO Terms
GO:0003823 antigen binding
GO:0002455 humoral immune response mediated by circulating immunoglobulin
GO:0019731 antibacterial humoral response
GO:0042571 immunoglobulin complex, circulating
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0006958 complement activation, classical pathway
GO:0050766 positive regulation of phagocytosis

STAR12A

1609

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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