IgG Antibody

//www.bio-rad-antibodies.com/mouse-igg-antibody-12004157.html?RenderPageSpecific=1
IgG Antibody gallery image 1

StarBright Blue 700 Secondary Antibodies

Enlarge
IgG Antibody gallery image 2

Schematic representation of a StarBright Blue 700 Label conjugated to an antibody. The light-absorbing monomer is excited at 470 nm and, via an efficient intramolecular transfer process, the energy is transmitted to a light-emitting monomer, which emits at 700 nm. An antibody is conjugated to linker monomers.

Enlarge
IgG Antibody gallery image 3

StarBright Blue 700 Secondary Antibodies in Multiplex Fluorescent Western Blotting. StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores as described in the table above. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.

* Stain-free gels can be used for total protein normalization in place of or in addition to housekeeping proteins (HKP)

Enlarge
IgG Antibody gallery image 4

StarBright Blue 700 Secondary Antibodies in Multiplex Fluorescent Western Blotting. Simultaneous detection of multiple proteins (ATG7, AKR1C2, and tubulin) on a single blot using fluorescent antibodies.

Enlarge
IgG Antibody gallery image 5

StarBright Blue 700 Secondary Antibodies in Multiplex Fluorescent Western Blotting to Detect Posttranslational Modifications. Composite blot image of triplex western blotting showing post-translational phosphorylation of a target protein. Multiplexing to detect unmodified target protein, the phospho-target, and a housekeeping protein all in one blot.

Enlarge
  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • IgG Antibody thumbnail image 5
  • Goat ANTI-MOUSE IgG StarBright™ Blue 700
  • Goat ANTI-MOUSE IgG StarBright™ Blue 700
  • Goat ANTI-MOUSE IgG StarBright™ Blue 700
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    12004158WBdatasheet pdfdatasheet pdf400 µl
    12004158
    12004157WBdatasheet pdfdatasheet pdf5 X 400µl
    12004157
    12004159WBdatasheet pdfdatasheet pdf80 µl
    12004159
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • StarBright Blue 700 Secondary Antibodies are ideal for fluorescent western blotting — either for the detection of a single target protein or for multiplex detection of several proteins on one blot, without stripping and reprobing. The StarBright Fluorophore is composed of a condensed polymer made up of multiple light-absorbing and -emitting monomers which provides an exceptionally bright signal (high quantum yield) compared to most traditional fluorophores.

      StarBright Blue 700 Fluorescent Secondary Antibodies can be used with traditional fluorophores like the DyLight 800 Fluorophore for multiplexing. In addition, StarBright Antibodies can be used with Bio-Rad's stain-free technology and/or the hFAB™ Rhodamine Anti-Housekeeping Primary Antibodies for protein normalization. These antibodies are optimized for use with the ChemiDoc™ MP Imaging System, permitting detection of up to three proteins in a single blot.

      Features and Benefits

      Easy multiplexing — StarBright Secondary Antibodies can be used with IRDye 800 or other common fluorophores to detect multiple proteins on the same blot
      Short exposure time — the exceptional brightness of the StarBright Fluorophore leads to shorter exposure times compared to traditional fluorescent dyes: seconds vs. minutes
      High signal-to-noise ratio — StarBright Secondary Antibodies emit in the red/far red, where there is minimal background (Exmax/Emmax = 470 nm/700 nm)
      Low nonspecific binding — StarBright Fluorophore is conjugated to highly cross-adsorbed antibodies

      Goat Anti-Mouse IgG StarBright Blue 700 has been cross-adsorbed against bovine, goat, human, rabbit and rat.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to StarBright Blue 700 - lyophilized
      Purified IgG conjugated to StarBright Blue 700 - lyophilized
      Purified IgG conjugated to StarBright Blue 700 - lyophilized
    • Reconstitution
      Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
      Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
      Resuspend contents of the tube in the indicated volume of distilled or deionized water and leave on ice for at least 30 min prior to use. Brief centrufugation (pulse spin for 2-3 sec at max speed in a tabletop microcentrifuge) may be used to collect the contents at the bottom of the tube. Do not centrifuge more than 10 sec.
    • Preparation
    • Antiserum Preparation
      Antiserum to Mouse IgG was raised by repeated immunisation of Goats with highly purified antigen.
    • Preservative Stabilisers
      >80% Sucrose
      <1% Polyethylene Glycol
      <0.04% 2-Methyl-2H-Isothiazol-3-One
      <10% Bovine Serum Albumin
      >80% Sucrose
      <1% Polyethylene Glycol
      <0.04% 2-Methyl-2H-Isothiazol-3-One
      <10% Bovine Serum Albumin
      >80% Sucrose
      <1% Polyethylene Glycol
      <0.04% 2-Methyl-2H-Isothiazol-3-One
      <10% Bovine Serum Albumin
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
      This product is photosensitive and should be protected from light
      DO NOT FREEZE the solubilized material
      1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
      This product is photosensitive and should be protected from light
      DO NOT FREEZE the solubilized material
      1 year at -20oC lyophilized; 6 months at +4oC after resuspension.
      This product is photosensitive and should be protected from light
      DO NOT FREEZE the solubilized material
    • Shelf Life
      Please see label for expiry date
      Please see label for expiry date
      Please see label for expiry date
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/2,5001/5,000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/2,5001/5,000
    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/2,5001/5,000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
      StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
      Note: StarBright Antibodies are not suitable for stripping and reprobing.
    • Western Blotting
      StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
      Note: StarBright Antibodies are not suitable for stripping and reprobing.
    • Western Blotting
      StarBright Blue 700 Secondary Antibodies can be used for multiplex fluorescent western blotting with the right combination of fluorophores. These fluorescent secondary antibodies can also be used to detect post-translational modifications like phosphorylation of a target protein.
      Note: StarBright Antibodies are not suitable for stripping and reprobing.
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/12004157.pdf

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Starbright Blue 700 WB 400 µl | 5 X 400µl | 80 µl
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
          STAR36D680GA
          ANTI-ACTIN hFAB™ Rhodamine Antibody12004163200 µlWB
          12004163
          ANTI-TUBULIN hFAB™ Rhodamine Antibody12004165200 µlWB
          12004165
          ANTI-GAPDH hFAB™ Rhodamine Antibody12004167200 µlWB
          12004167
          ANTI-ACTIN hFAB™ Rhodamine Antibody1200416440 µlWB
          12004164
          ANTI-TUBULIN hFAB™ Rhodamine Antibody1200416640 µlWB
          12004166
          ANTI-GAPDH hFAB™ Rhodamine Antibody1200416840 µlWB
          12004168
          Goat ANTI-RABBIT IgG StarBright™ Blue 70012004161400 µlWB
          12004161
          Goat ANTI-RABBIT IgG StarBright™ Blue 700120041605 X 400µlWB
          12004160
          Goat ANTI-RABBIT IgG StarBright™ Blue 7001200416280 µlWB
          12004162
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
          STAR36D800GA
          ANTI-ACTIN hFAB™ Rhodamine Antibody12004163200 µlWB
          12004163
          ANTI-TUBULIN hFAB™ Rhodamine Antibody12004165200 µlWB
          12004165
          ANTI-GAPDH hFAB™ Rhodamine Antibody12004167200 µlWB
          12004167
          ANTI-ACTIN hFAB™ Rhodamine Antibody1200416440 µlWB
          12004164
          ANTI-TUBULIN hFAB™ Rhodamine Antibody1200416640 µlWB
          12004166
          ANTI-GAPDH hFAB™ Rhodamine Antibody1200416840 µlWB
          12004168
          Goat ANTI-RABBIT IgG StarBright™ Blue 70012004161400 µlWB
          12004161
          Goat ANTI-RABBIT IgG StarBright™ Blue 700120041605 X 400µlWB
          12004160
          Goat ANTI-RABBIT IgG StarBright™ Blue 7001200416280 µlWB
          12004162
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
          STAR36D800GA
          ANTI-ACTIN hFAB™ Rhodamine Antibody12004163200 µlWB
          12004163
          ANTI-TUBULIN hFAB™ Rhodamine Antibody12004165200 µlWB
          12004165
          ANTI-GAPDH hFAB™ Rhodamine Antibody12004167200 µlWB
          12004167
          ANTI-ACTIN hFAB™ Rhodamine Antibody1200416440 µlWB
          12004164
          ANTI-TUBULIN hFAB™ Rhodamine Antibody1200416640 µlWB
          12004166
          ANTI-GAPDH hFAB™ Rhodamine Antibody1200416840 µlWB
          12004168
          Goat ANTI-RABBIT IgG StarBright™ Blue 70012004161400 µlWB
          12004161
          Goat ANTI-RABBIT IgG StarBright™ Blue 700120041605 X 400µlWB
          12004160
          Goat ANTI-RABBIT IgG StarBright™ Blue 7001200416280 µlWB
          12004162

          Recommended Positive Controls

            Histology Controls

              Write your review

              • View more products with "IgG" specificity.
               

              You may also be interested in...