High Mobility Group Protein B1 Antibody | HMG1-5H6

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High Mobility Group Protein B1 Antibody | HMG1-5H6 gallery image 1

Western Blotting analysis of proteins and cell line lysates probed with Hamster anti Mouse high mobility group protein B1 (MCA5765)

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High Mobility Group Protein B1 Antibody | HMG1-5H6 gallery image 2

Immunofluorescence staining of PFA fixed permeablized NIH-3T3 cells with Hamster anti Mouse high mobility group protein B1 (MCA5765), signal was visualized using Goat anti Hamster IgG:biotin followed by streptavidin:AlexaFluor594

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High Mobility Group Protein B1 Antibody | HMG1-5H6 gallery image 3

Staining of permeablized RAW264.7 cells with Hamster anti Mouse high mobility group protein B1 (MCA5765) followed by Goat anti Hamster IgG:PE

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  • Hamster anti High Mobility Group Protein B1
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    HMG1-5H6
  • Isotype
    IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA5765GAF, IF, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA5765GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Hamster anti High Mobility Group Protein B1 antibody, clone HMG1-5H6 specifically recognizes High mobility group protein B1 (HMGB1), a ubiquitously expressed nuclear DNA binding protein, and one of a newly emerging group of alarmins, which acts as a stabilizer of nucleosome formation, and facilitates transcription factor binding, by bending DNA.

      HMGB1 is one of the signature danger signals of endogenous cellular injury, and is released outside the cell by necrotic and inflammatory cells to act as a chemoattractant for immature dendritic cells (DCs), promoting their maturation. DCs can also secrete HMGB1, promoting proliferation and Th1 polarization of interacting T cells. Cellular injury resulting in necrosis, leads to passive HMGB1 release, and microbes or pro-inflammatory cytokines may later stimulate active release from APCs.

      HMGB1 is emerging as a prime specific marker and regulator of necrotic cell death, possibly through the PI3KC3-MEK-ERK pathway. It interacts directly with the autophagy protein Beclin-1, and binds to receptors, such as RAGE on endothelial cells, and Toll-like receptors on macrophages. Studies identifying microtubule-associated protein 1 light chain 3 (LC3) lipidation and redistribution, coupled with the accumulation of autophagosomes and autolysosomes, have shown an important role for HMGB1 release in sustaining autophagy. Studies have also shown that HMGB1 released after chemotherapy treatment is a critical regulator of autophagy, and a potential drug target for therapeutic interventions in leukemia.

      HMGB1 is increasingly recognized as an important protein in medical research. It is angiogenic and promotes cardiac stem cell growth and differentiation, it may act as an adjuvant or assist in tissue repair, and is also a prototypical damage-associated molecular pattern molecule (DAMP) which co-precipitates with CD24 and is associated with the hallmarks of cancer. Accelerated levels of HMGB1 are seen in
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA5765GA detects a band of approximately 30kDa in mouse NIH-3T3, rat NRK, hamster BHK, and HeLa cell lysates in the presence of 2ME, and bands of 30kDa (red) and 27kDa (ox) in the absence of 2ME.
    • Instructions For Use

    Additional High Mobility Group Protein B1 Antibody Formats

    Formats Clone Applications Sizes available
    High Mobility Group Protein B1 Antibody : Purified HMG1-5H6 F, IF, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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