Glutathione Peroxidase 4 Antibody

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  • Rabbit anti Mouse Glutathione Peroxidase 4 (Ct)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1362E, WBdatasheet pdfdatasheet pdf50 µg
    AHP1362
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Mouse glutathione peroxidase 4 antibody detects the carboxy-terminal region of mouse glutathione peroxidise 4 (GPx4) also known as phospholipid hydroperoxidase (PHGPx). GPx4 is a 20-22 kDa selenoprotein that has an important role in preventing lipid peroxidation, and is thought to protect biomembranes against oxidative stress.

      GPx4 is expressed in high levels in testes, suggesting a role in male fertility. It has also been identified as an enzymatically inactive structural protein of the mitochondrial capsule of sperm. GPx4 has also been proposed as a modulator of various eicosanoids, which serve as inflammatory lipid signalling molecules.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      RatExpected from Sequence
      Guinea PigExpected from Sequence
      HumanExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to mouse Glutathione peroxidase 4 (CT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.01%Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to amino acids near the carboxyl terminus of mouse glutathione peroxidase 4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      multicellular organismal development
      glutathione peroxidase activity
      nuclear envelope
      mitochondrial inner membrane
      cytosol
      oxidation-reduction process
      phospholipid-hydroperoxide glutathione peroxidase activity
      spermatogenesis
      chromatin organization
      response to oxidative stress
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/70001/30000
      Western Blotting1/5001/3000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1362 detects a band of approximately 19kDa in mouse testis cell lysates.
    • Instructions For Use

    Additional Glutathione Peroxidase 4 Antibody Formats

    Formats Applications Sizes available
    Glutathione Peroxidase 4 Antibody : Purified E, WB 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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