G Protein Alpha 0 Antibody

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Synthetic G Protein Alpha 0 detected with Rabbit anti Mouse G Protein Alpha 0 (4737-1010)

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  • Rabbit anti Mouse G Protein Alpha 0
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4737-1010E, WB*datasheet pdfdatasheet pdf1 ml
    4737-1010
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse G Protein alpha 0 antibody recognizes the alpha subunit of murine Guanine nucleotide-binding protein G(o). Guanine nucleotide-binding protein G(o) is a 354 amino acid membrane associated protein of ~40kDa forming a heterotrimeric protein complex with β and γ subunits. Two isoforms of the α subunit are generated by alternative splicing with a single amino acid substitution in the immunogen sequence (a lysine for an asparagine at position 346), it is not known whether this substitution affects the binding to isoform 2.

      Rabbit anti Mouse G Protein alpha 0 antibody has been used successfully for the detection of G(o) in Drosophila by western blotting (Guo et al. 2011).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Drosophilayes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the amino acids 345 - 354 of the native isoform 1 molecule conjugated to Keyhole Limpet Haemocyanin.
    • Peptide Sequence
      {C-}A-N-N-L-R-G-C-G-L-Y
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted.
      Avoid repeated freezing and thawing as this may denature the antibody.
      Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      intracellular
      signal transducer activity
      heterotrimeric G-protein complex
      regulation of heart contraction
      GTPase activity
      locomotory behavior
      dopamine receptor signaling pathway
      GTP binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/5001/1000
      Western Blotting(1)
      (1)
      This antibody detects band at 39 kDa when used under reducing conditions against rat brain.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional G Protein Alpha 0 Antibody Formats

    Formats Applications Sizes available
    G Protein Alpha 0 Antibody : Purified E, WB* 1 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

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