G-CSF Antibody

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  • Rabbit anti Mouse G-CSF:Biotin
  • Rabbit anti Mouse G-CSF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM28E, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AAM28
    AAM28BE, WBdatasheet pdfdatasheet pdf50 µg
    AAM28B
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse G-CSF antibody recognizes mouse granulocyte colony stimulating factor, also known as G-CSF.

      G-CSF stimulates the proliferation and differentiation of granulocyte progenitors in the bone marrow.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to Biotin - lyophilised
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 1.0 ml sterile PBS containing 0.1% Bovine Serum Albumin. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
      Reconstitute with 0.1 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Antiserum Preparation
      Antisera to mouse G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      None present
      None Present
    • Immunogen
      Recombinant mouse G-CSF.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 50 ug/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. The addition of 0.09% sodium azide is recommended for long term storage.
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage, the addition of 0.09% sodium azide is recommended. N.B. For functional studies, do not use azide.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of reconstitution.
    • GO Terms
      growth factor activity
      immune response
      granulocyte differentiation
      extracellular space
      cytokine activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.15ug/ml0.30ug/ml
      Western Blotting0.1ug/ml0.2ug/ml
    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5ug/ml
      Functional Assays0.005ug/ml0.010ug/ml
      Western Blotting0.1ug/ml0.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional G-CSF Antibody Formats

    Formats Applications Sizes available
    G-CSF Antibody : Purified E, FN, WB 0.1 mg
    G-CSF Antibody : Biotin E, WB 50 µg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
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          Recommended Positive Controls

            Histology Controls

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