Forssman Glycosphingolipid Antibody | FOM-1

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  • Rat anti Mouse Forssman Glycosphingolipid
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    FOM-1
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0500-0098C, Pdatasheet pdfdatasheet pdf0.5 ml
    0500-0098
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • 0500-0098 recognises Forssman Glycosphingolipid (FO GSL).

      FO GSL is a glycosphingolipid differentiation antigen that is present on macrophages in the red pulp of the spleen, and the medulla of inguinal and axial lymph nodes. FO GSL is also present on reticular cells in the T-cell area of the spleen and lymph nodes. A subpopulation of FOM-1 positive macrophages also appear in the peritoneal cavity in response to inflammatory stimuli. Monocytes, granulocytes and other macrophages in lymphoid and non-lymphoid organs are not stained by FOM-1.
      This antibody also recognises a marker present on sheep erythrocytes.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Sheepyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM - liquid.
    • Reconstitution
    • Preparation
      Purified IgM prepared by dialysis and gel filtration
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Forsssman glycosphingolipid
    • Purity
    • Approx. Protein Concentrations
      0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen1/1001/400
      Immunohistology - Paraffin1/100

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Mouse spleen
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Forssman Glycosphingolipid Antibody Formats

    Formats Clone Applications Sizes available
    Forssman Glycosphingolipid Antibody : Purified FOM-1 C, P 0.5 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgM:Alk. Phos.STAR116A0.1 mgE, WB
      STAR116A
      Goat anti Rat IgM:Alk. Phos.STAR116AB0.5 mgE, WB
      STAR116AB
      Goat anti Rat IgM:Biotin3020081 mgC, E, IB
      302008
      Goat anti Rat IgM:FITCSTAR116F0.5 mgF
      STAR116F
      Goat anti Rat IgM:HRPSTAR116P0.5 mgE, WB
      STAR116P
      Goat anti Rat IgM:RPE3020090.5 mgF
      302009

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Mouse spleen

              References

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