Fibronectin Antibody

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Rabbit anti Murine fibronectin antibody (4470-4339) used to demonstrate fibronectin deposition in the striatum of rats in a transient focal ischemia model.
Image caption:
Triple fluorescence staining of fibronectin (FN), collagen IV (Coll) and STL in the striatum (A-A”‘) of non-treated rats 7 days after transient focal cerebral ischemia. Dense Cy2-immunolabeling of FN (A’) was seen in the ischemia-affected region simultaneously showing strongly up-regulated collagen IV-immunoreactivity (A) intermingled by lectin-histochemically stained microglia/macrophages (A”, STL color-coded in blue). Concomitantly, STL revealed some apparently healthy endothelial cells in the surrounding tissue. Merging the staining patterns (A”‘) revealed that the region with up-regulated collagen IV was somewhat more extended than the regions with enhanced signals for fibronectin and microglia/macrophages. Scale bars: in A” (also valid for A and A’) = 100 μm.

From: Michalski D, Pitsch R, Pillai DR, Mages B, Aleithe S, Grosche J, et al. (2017) Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors. PLoS ONE 12(4): e0174996.

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Fibronectin Antibody gallery image 2

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Rabbit anti Murine fibronectin antibody (4470-4339) used to demonstrate fibronectin deposition at the neocortex of rats in a transient focal ischemia model.
Image caption:
Triple fluorescence staining of fibronectin (FN), collagen IV (Coll) and STL at the neocortex (B-B”‘) of non-treated rats 7 days after transient focal cerebral ischemia. Fibronectin staining in the neocortex (B’) elucidated this protein not only in the neuropil, but also in numerous somata within the ischemia-affected tissue, which displays only partially up-regulated collagen IV (B) and persisting STL-stained vessels (B”). Merging the staining patterns (B”‘) showed some fibronectin-containing cells with STL-labeling. Scale bars: in B” (also valid for B and B’) = 50 μm.

From: Michalski D, Pitsch R, Pillai DR, Mages B, Aleithe S, Grosche J, et al. (2017) Delayed histochemical alterations within the neurovascular unit due to transient focal cerebral ischemia and experimental treatment with neurotrophic factors. PLoS ONE 12(4): e0174996.

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  • Rabbit anti Mouse Fibronectin
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    4470-4339E, IF, WBdatasheet pdfdatasheet pdf1 ml
    4470-4339
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Mouse Fibronectin antibody recognizes fibronectin, a 460-500 kDa glycoprotein involved in tissue organisation and wound repair.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Chickenyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Ig fraction - lyophilised
    • Reconstitution
      Reconstitute in 1 ml of sterile distilled water. For long term storage the addition of a preservative is recommended.
    • Preparation
      Ig fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      None present
    • Immunogen
      Purified murine fibronectin.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Ammonium bicarbonate
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      cell-substrate junction assembly
      basement membrane
      cell-matrix adhesion
      acute-phase response
      peptidase activator activity
      regulation of cell shape
      apical plasma membrane
      wound healing
      angiogenesis
      heparin binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Western Blotting1/3001/400

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Fibronectin Antibody Formats

    Formats Applications Sizes available
    Fibronectin Antibody : Purified E, IF, WB 1 ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              References

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