Farnesyl Diphosphate Synthetase Antibody | 2122

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Western Blot analysis of various adult mouse tissues probed with HCA012, followed by Goat anti Human IgG F(ab)2: HRP (0500-0099)

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  • Human anti Mouse Farnesyl Diphosphate Synthetase
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  • Product Type
    Monoclonal Antibody
  • Clone
    2122
  • Isotype
    HuCAL Fab bivalent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA012C, E, WBdatasheet pdfdatasheet pdf0.1 mg
    HCA012
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Anti mouse farnesyl diphosphate synthetase antibody recognizes the C-terminal 116 amino acids of farnesyl diphosphate synthetase.
      Due to high homology, the antibody may cross-react with the farnesyl diphosphate synthetase of other species. The cross-species identity is: rat 98%, bovine 89%, human 89%, orang utan 89%, chimpanzee 89%, chicken 86%.

      The mKIAA1293 protein, also known as farnesyl diphosphate synthetase, catalyzes the formation of a key cellular intermediate in isoprenoid metabolic pathways. The sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphate, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate is catalyzed to ultimately yield the product farnesyl pyro-phosphate. Beside the isoprene and cholesterol biosynthesis, the enzyme seems to be active in the fibroblast growth factor (FGF)-mediated signalling as a modulator of the cellular response to FGF treatment. The cytoplasmic enzyme forms a homodimer and belongs to the FPP/GGPP synthetase family.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
    • Reconstitution
      Reconstitute with 100ul distilled water
    • Preparation
      Metal-chelate affinity chromatography
    • Preservative Stabilisers
      None present
    • Immunogen
      A fusion protein of a domain murine farnesyl diphosphate synthetase (amino acid residues 238-353, MW 13 kDa) expressed in E. coli.
    • Purity
    • Approx. Protein Concentrations
      Antibody concentration 1.0 mg/ml following reconstitution
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
    • Shelf Life
      6 months from date of reconstitution.
    • GO Terms
      metal ion binding
      dimethylallyltranstransferase activity
      isoprenoid biosynthetic process
      mitochondrion
      geranyltranstransferase activity
      cholesterol biosynthetic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.

      This antibody was developed by Bio-Rad, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany, in collaboration with the Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818 JAPAN.

      WB data kindly provided by the Kazusa DNA Research Institute, Kisarazu, Japan.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA2ug/ml
      Immunohistology - Frozen6.8ug/ml
      Western Blotting0.5ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Farnesyl Diphosphate Synthetase Antibody Formats

    Formats Clone Applications Sizes available
    Farnesyl Diphosphate Synthetase Antibody : Purified 2122 C, E, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Mouse anti Penta Histidine Tag:HRPMCA5995P125 µlE, WB
      MCA5995P
      Mouse anti c-MycMCA22001 mgC, E, F *, P, WB*
      MCA2200

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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