Recombinant Murine epidermal growth factor (EGF) is a ~6.0 kDa globular protein with a primary role in the stimulation of epidermal and epithelial cell proliferation. It also inhibits gastric secretion, is involved in wound healing and intestinal development. It activates the MAPK pathway and inhibits c-Myc-mediated apoptosis.
Purified recombinant protein - lyophilised
Reconstitute with 0.5ml distilled water
Purified recombinant EGF, expressed in E. coli
The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is <0.1ng/ml, corresponding to a specific activity of >1x107units/mg.
>98% by SDS PAGE and HPLC analysis
Approx. Protein Concentrations
6.0kD (53 amino acid residues)
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. After reconstitution store at -20oC only. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.