EGF exhibits growth stimulating properties on various epidermal and epithelial tissues, both in vivo and in vitro.
Purified natural EGF - lyophilised
Reconstitute with 1.0ml phosphate buffered saline (PBS). Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. N.B. For functional studies do not add sodium azide
Purified from the submaxillary glands of male mice, using a modification of the method of Savage and Cohen(1).
>95% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml after reconstitution
Protein Molecular Weight
Reagents In The Kit
Preparing The Antibody
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.