EGF

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  • Mouse EGF
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Purified Protein
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    EGF1FNdatasheet pdfdatasheet pdf1 mg
    EGF1
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • EGF exhibits growth stimulating properties on various epidermal and epithelial tissues, both in vivo and in vitro.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified natural EGF - lyophilised
    • Reconstitution
      Reconstitute with 1.0ml phosphate buffered saline (PBS).
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
      N.B. For functional studies do not add sodium azide
    • Preparation
      Purified from the submaxillary glands of male mice, using a modification of the method of Savage and Cohen(1).
    • Preservative Stabilisers
      None present
    • Purity
      >95% by SDS PAGE
    • Approx. Protein Concentrations
      Total protein concentration 1.0mg/ml after reconstitution
    • Protein Molecular Weight
      6,045Da
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      activation of MAPKK activity
      epidermal growth factor receptor signaling pathway
      plasma membrane
      extracellular space
      calcium ion binding
      peptidyl-tyrosine phosphorylation
      integral to membrane
      growth factor activity
      positive regulation of cerebellar granule cell precursor proliferation
      mammary gland alveolus development
      branching morphogenesis of a tube
      regulation of peptidyl-tyrosine phosphorylation
      epidermal growth factor receptor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Functional Assays

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional EGF Formats

    Formats Applications Sizes available
    EGF : Purified FN 1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Further Reading

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