Dendritic/Interdigitating Cells Antibody | MIDC-8

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Dendritic/Interdigitating Cells Antibody | MIDC-8 gallery image 1

Published customer image:
Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 used for the detection of dendritic cells by immunohistochemistry on cryostat sections.
Image caption:
Loss of MHC class II expression and bone-marrow-derived stromal cell elements in the thymus of IL-2-/- mice. Frozen sections of thymuses from 5-week-old SPF IL-2+/+ (+/+) and IL-2 -/- (-/-) mice were stained with the anti-macrophage-specific antibodies Mac-1 (A and B) and F4/80 (C and D), and with the anti-thymic dendritic-cell antibody MIDC-8 (E and F) and the anti-MHC class II-specific antibody Y-3P (G and H). Bound antibody was visualized as described in the legend to Fig. 4. The arrow heads in E and F identify positively stained dendritic cells, m: medulla; c: cortex. Magnification: 200.

From: Tannishtha Reya, Hamid Bassiri, Renée Biancaniello, and Simon R. Carding, “Thymic Stromal-Cell Abnormalities and Dysregulated T-Cell Development in IL-2-Deficient Mice,” Developmental Immunology, vol. 5, no. 4, pp. 287-302, 1998.

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  • Rat anti Mouse Dendritic/Interdig. Cells
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  • Product Type
    Monoclonal Antibody
  • Clone
    MIDC-8
  • Isotype
    IgG2a
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA948Cdatasheet pdfdatasheet pdf2 ml
    MCA948
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 recognizes an antigen in the cytoplasm of rat dendritic cells as distinct spots near the nucleus.

      Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 does not stain thymic epithelial cells which are the major contaminants in thymic dendritic cell preparations. The antigen is also absent in blood cells, bone marrow cells and macrophages isolated from the peritoneal cavity.

      The antigen recognized by Rat anti Mouse dendritic/interdigitating cells antibody, clone MIDC-8 is found in interdigitating cells in T cell dependent areas of secondary lymphoid organs, the medulla of thymus, veiled cells, and very weakly in Langerhans cells (Breel et al. 1987).
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Tissue Culture Supernatant - liquid
    • Reconstitution
    • Preparation
      Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Mouse lymph node stroma.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Fusion Partners
      Spleen cells from immunized rats were fused with Sp2/0 myeloma cells.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/25

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate positive/negative controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Dendritic/Interdigitating Cells Antibody Formats

    Formats Clone Applications Sizes available
    Dendritic/Interdigitating Cells Antibody : S/N MIDC-8 C 2 ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunofluorescence
                Immunohistology - Frozen
                Immunohistology - Paraffin

              References

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