Dectin-2 Antibody | D2.11E4

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Dectin-2 Antibody | D2.11E4 gallery image 1

Mouse spleen stained wiith Rat anti Mouse Dectin-2 (MCA2415)

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Assessment of dectin-2 function on inflammatory cells. A) Flow-cytometric plots showing expression of dectin-2 on the surface of inflammatory neutrophils (Ly-6G+) and monocyte/MØ (Ly-6G-). Data is pre-gated on Ly-6B.2+ cells and is representative of results obtained with pooled cells from 3 independent experiments. B) Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/MØ from dectin-1-deficient mice. All bars show mean±95% confidence intervals of dectin-2-blocked cells relative to isotype control treated cells (100%, dotted line) from 3 independent experiments (raw representative data, which includes the isotype control data, from one of the 3 independent experiments are shown in Figure S2). Data were analysed by two-way ANOVA, two assess the differences caused by complement opsonization (‘C’) or the use of different cell lineages (‘L’). Samples in which the 95% confidence intervals do not overlap with the mean isotype control are specific indicated with a # symbol.

From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.

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Dectin-2 Antibody | D2.11E4 gallery image 3

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Inhibition of dectin-1 and -2-mediated fungal recognition by prior complement opsonization. A) Representative flow cytometric plots of dectin-2 surface expression on NIH3T3 retrovirally transduced with dectin-2 (left panel) and both dectin-2 and FceR? chain (right panel). B) Serum-opsonization inhibits the recognition of zymosan by both dectin-1 and dectin-2. NIH3T3 retrovirally transduced as indicated in the legend were incubated with zymosan that had been pre-incubated with serum at increasing concentrations and the interaction was monitored by flow cytometry. Data represents the mean±SEM of triplicates from a representative of 2 independent experiments. Data in (D) was analyzed by two-way ANOVA, with the significant Interaction statistics demonstrating the reduced binding of zymosan to the transduced cells after prior incubation with increasing concentrations of serum.

From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.

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Dectin-2 Antibody | D2.11E4 gallery image 4

Published customer image:
Assessment of dectin-2 function on inflammatory cells. Assessment of the impact of blockade of dectin-2 (D2.11E4) on the recognition (upper graphs) and response to (lower graphs) zymosan of inflammatory neutrophils and monocyte/MØ. Data represents mean±SEM of triplicates from a representative of 3 independent experiments.

From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.

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  • Rat anti Mouse Dectin-2:Low Endotoxin
  • Rat anti Mouse Dectin-2
  • Rat anti Mouse Dectin-2
  • Rat anti Mouse Dectin-2
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    D2.11E4
  • Isotype
    IgG2a
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2415GAC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2415GA
    MCA2415C, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2415
    MCA2415ELC, F, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2415EL
    MCA2415TC, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2415T
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rat anti Mouse Dectin-2 antibody, clone D2.11E4 recognizes mouse DC-associated C-type lectin-2 (Dectin-2), a type II transmembrane protein that is a member of the C-type lectin superfamily. In mouse, dectin-2 is predominantly expressed on tissue macrophages and some dendritic cells. Clone D2.11E4 does not stain brain microglia or meningeal macrophages.

      Significant expression of Dectin-2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin-2 but transient up-regulation of expression has been demonstrated on monocytes at sites of inflammation.

      The function of dectin-2 has not been fully determined but studies suggest a possible role in immune-surveillance.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      None present
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
    • Immunogen
      Dectin-2 (extracellular domain) Fc Fusion protein.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      positive regulation of I-kappaB kinase/NF-kappaB cascade
      sugar binding
      innate immune response
      defense response to fungus
      positive regulation of cytokine secretion
      protein binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/100
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25 Pack Size: 0.25 mg, 25 µg
      1/50 Pack Size: 0.1 mg
      1/50Pack Size: 0.25 mg, 25 µg
      1/100Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25 Pack Size: 0.25 mg, 25 µg
      1/50 Pack Size: 0.1 mg
      1/50Pack Size: 0.25 mg, 25 µg
      1/100Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      1/25 Pack Size: 0.25 mg, 25 µg
      1/50 Pack Size: 0.1 mg
      1/50Pack Size: 0.25 mg, 25 µg
      1/100Pack Size: 0.1 mg
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Dectin-2 Antibody Formats

    Formats Clone Applications Sizes available
    Dectin-2 Antibody : Purified D2.11E4 C, F, IP 0.25 mg | 0.1 mg | 25 µg
    Dectin-2 Antibody : Low Endotoxin D2.11E4 C, F, IP 0.5 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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            Histology Controls

              References

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