Dectin-1 Antibody | 2A11

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Dectin-1 Antibody | 2A11 gallery image 1

Flow cytometric staining of mouse peripheral blood granulocytes with RPE conjugated Rat anti Mouse Beta-glucan Receptor (MCA2289PE)

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Dectin-1 Antibody | 2A11 gallery image 2

Flow cytometric staining of mouse peripheral blood granulocytes with Rat anti Mouse Beta-glucan Receptor: Alexa Fluor®647 (MCA2289A647)

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Flow cytometric staining of mouse peripheral blood granulocytes with FITC conjugated Rat anti Mouse Beta-glucan Receptor (MCA2289FA)

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Flow cytometric staining of mouse peripheral blood granulocytes with biotin conjugated Rat anti Mouse Beta-glucan Receptor (MCA2289B)

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Flow cytometric staining of mouse peripheral blood monocytes with low endotoxin Rat anti Mouse Beta-glucan Receptor (MCA2289EL)

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Flow cytometric staining of mouse peritoneal macrophages with FITC conjugated Rat anti Mouse Beta-glucan Receptor (MCA2289FB)

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Dectin-1 Antibody | 2A11 gallery image 7

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Low power

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Dectin-1 Antibody | 2A11 gallery image 8

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Medium power

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Dectin-1 Antibody | 2A11 gallery image 9

Immunoperoxidase staining of a mouse skin cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). Low power

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Dectin-1 Antibody | 2A11 gallery image 10

Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody (STAR72). High power

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Dectin-1 Antibody | 2A11 gallery image 11

Published customer image:
Rat ani Mouse Dectin 1 antibody, clone 2A11 (MCA2289) used for the evaluation of Dectin-1 expression on neutrophils by flow cytometry.Image caption:
Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/Mø. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean±95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (‘C’) and the use of different fungal particle used (‘F’) were assessed by Two-way ANOVA (‘I’ = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.

From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781

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Dectin-1 Antibody | 2A11 gallery image 12

Published customer image:
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of dectin-1 expression on wild type and knockout mouse macrophages by flow cytometry.
Image caption:
Dectin-1 response and expression is Ae2-dependent.
(A and B) Tnfα mRNA expression relative to Hprt in Ae2wt/wt and Ae2Δ/Δ macrophages stimulated for 6h with Zymosan (5 ppc) (A) or PAM3CK4 (B) (n = 8–10, ** p<0.01, Mann-Whitney test). (C) Ae2 mRNA expression relative to Hprt by WT macrophages stimulated for 6h with Zymosan (Zym, 5 ppc) or PAM3CK4 (100 ng/ml) (n = 8–12, * p<0.05, *** p<0.001, Mann-Whitney test). (D) Representative flow cytometry histogram with quantification by mean fluorescence intensity (MFI) of Dectin-1 expression on cell surface of Ae2wt/wt and Ae2Δ/Δ macrophages (n = 8, *** p<0.001, Mann-Whitney test). (E) Representative flow cytometry histogram and MFI of CD64 expression on cell surface of Ae2wt/wt and Ae2Δ/Δ macrophages (n = 5).

From: Urso K, Charles JF, Shull GE, Aliprantis AO, Balestrieri B (2016)
Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS ONE 11(7): e0158893.

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Dectin-1 Antibody | 2A11 gallery image 13

Published customer image:
Rat anti Mouse dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of dectin-1 expression on wild type and treated mouse macrophages by flow cytometry.
Image caption:
Pharmacological alkalinization of pHi inhibits Dectin-1 expression.
(A) Dectin-1 expression relative to Hprt by wild-type macrophages treated with bafilomycin A (Baf A, 25 nM) or DMSO control for 6 and 16 h. (B) Representative flow cytometry histogram and (C) quantification by MFI showing decreased Dectin-1 in wild-type macrophages treated with Baf A for 6 and 16 h compared to DMSO control (n = 6, ** p<0.01, Mann-Whitney test).

From: Urso K, Charles JF, Shull GE, Aliprantis AO, Balestrieri B (2016)
Anion Exchanger 2 Regulates Dectin-1-Dependent Phagocytosis and Killing of Candida albicans.
PLoS ONE 11(7): e0158893.

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Dectin-1 Antibody | 2A11 gallery image 14

Published customer image:
Rat anti Mouse Dectin-1 antibody, clone 2A11 (MCA2289) used for the evaluation of surface dectin-1 expression by murine peritoneal and bone marrow derived macrophages by flow cytometry.
Image caption:
Murine primary macrophages including peritoneal macrophages and BMDM express similar levels of the BG receptor dectin-1 and exhibit the same ability to respond to Sc BG extracts.
(A-B) Freshly isolated 4-days thioglycollate-elicited peritoneal macrophages (TEPM), resident peritoneal macrophages (RPM) and BMDM from WT and Clec7a-/- C57Bl/6, and DBA/2 mice, and the macrophages cell line RAW-Blue™, used as control, were assessed for surface expression of dectin-1 by flow cytometry (MACSQuant®, Miltenyi Biotech, Germany). Doublets of cells were excluded at the beginning of the acquisition and a 7-AAD staining was used to discriminate death cells. The surface expression of dectin-1 was determined for each type of primary macrophages on CD115+ cells. (A) Data are expressed as the frequency of dectin-1+ among CD115+ cells. (B) The mean of fluorescence intensity (MFI) of dectin-1 was established on CD115+ cells. Results are presented as the mean ± SD MFI of dectin-1 corrected by either the MFI of isotype control for RAW macrophages, or the MFI of Clec7a-/- C57Bl/6 for WT C57Bl/6 or DBA/2 primary macrophages. (C) Remaining TEPM and BMDM from WT and Clec7a-/- C57Bl/6 mice isolated from these experiments were subcultured and stimulated with 100 μg/mL of crude or enriched Sc BG CW extracts (BG15, BG65 or BG75) or zymosan for 8 h (37°C, 5% CO2). Supernatants were collected immediately after incubation and were assessed for TNFα production by ELISA. Results are shown as the mean ± SD. All data are representative of three mice per group from three independent experiments performed in triplicate. *p <0.05 (two-tailed unpaired Student's t-test); mean values not sharing the same letter are significantly different.

From: Walachowski S, Tabouret G, Foucras G (2016) Triggering Dectin-1-Pathway Alone Is Not Sufficient to Induce Cytokine Production by Murine Macrophages. PLoS ONE 11(2): e0148464.

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  • Rat anti Mouse Dectin-1
  • Rat anti Mouse Dectin-1
  • Rat anti Mouse Dectin-1:RPE
  • Rat anti Mouse Dectin-1:RPE
  • Rat anti Mouse Dectin-1
  • Rat anti Mouse Dectin-1:Biotin
  • Rat anti Mouse Dectin-1:Alexa Fluor® 647
  • Rat anti Mouse Dectin-1:Alexa Fluor® 647
  • Rat anti Mouse Dectin-1:FITC
  • Rat anti Mouse Dectin-1:Low Endotoxin
  • Rat anti Mouse Dectin-1:Biotin
  • Rat anti Mouse Dectin-1:FITC
  • Rat anti Mouse Dectin-1:FITC
  • Rat anti Mouse Dectin-1:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2A11
  • Isotype
    IgG2b
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2289GAC *, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA2289GA
    MCA2289BFdatasheet pdfdatasheet pdf0.1 mg
    MCA2289B
    MCA2289FFdatasheet pdfdatasheet pdf0.1 mg
    MCA2289F
    MCA2289C *, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA2289
    MCA2289ELC *, F, FN, IPdatasheet pdfdatasheet pdf0.5 mg
    MCA2289EL
    MCA2289FBFdatasheet pdfdatasheet pdf0.5 mg
    MCA2289FB
    MCA2289PEFdatasheet pdfdatasheet pdf100 Tests
    MCA2289PE
    MCA2289A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2289A647
    MCA2289TC *, F, IPdatasheet pdfdatasheet pdf25 µg
    MCA2289T
    MCA2289BTFdatasheet pdfdatasheet pdf25 µg
    MCA2289BT
    MCA2289FTFdatasheet pdfdatasheet pdf25 µg
    MCA2289FT
    MCA2289PETFdatasheet pdfdatasheet pdf25 Tests
    MCA2289PET
    MCA2289A647TFdatasheet pdfdatasheet pdf25 Tests/0.25ml
    MCA2289A647T
    MCA2289FAFdatasheet pdfdatasheet pdf50 µg
    MCA2289FA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rat anti Mouse Dectin-1 antibody, clone 2A11 recognizes murine beta-glucan receptor, also known as Dectin-1.Dectin-1 is predominantly expressed by cells of the monocyte/macrophage and neutrophil lineages, but also at lower levels by dendritic cells and a subpopulation of T cells.

      As a major leucocyte receptor for beta-glucan this molecule may have a key role in the immunomodulatory effects of beta-glucans and in the host response to fungal pathogens. Dectin-1 may stimulate reactive oxygen production in macropahges via the protein tyrosine kinase known as Syk.

      Rat anti Mouse Dectin-1 antibody, clone 2A11 inhibits the binding of zymosan to macrophages via the beta-glucan receptor.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Pack Size: 100 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
      Pack Size: 25 TestsPurified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
      Purified IgG conjugated to Alexa Fluor® 647- liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
    • Reconstitution
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
      Pack Size: 100 TestsReconstitute with 1 ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Preservative Stabilisers
      0.09%Sodium Azide
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
      0.09%Sodium Azide
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      None present
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      0.09%Sodium Azide
      1%Bovine Serum Albumin
    • Immunogen
      Dectin-1 transfected NIH3T3 cells and recombinant soluble Dectin-1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      IgG concentration 0.05 mg/ml
      IgG concentration 0.05 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.1 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
      Pack Size: 0.1 mg, 50 µg, 25 µgIgG concentration 0.1 mg/ml
      Pack Size: 0.5 mgIgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Fischer rats were fused with cells of the rat Y3 myeloma cell line
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mg, 0.5 mg, 25 µgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mg, 0.5 mg, 25 µgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mg, 0.5 mg, 25 µgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mg, 0.5 mg, 25 µgStore at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgStore at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of reconstitution.
      18 months from date of reconstitution.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      12 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      phagocytosis, engulfment
      positive regulation of tumor necrosis factor production
      cell-cell adhesion
      inflammatory response
      innate immune response
      external side of plasma membrane
      1,3-beta-D-glucan receptor activity
      cell surface pattern recognition receptor signaling pathway
      phagocytosis, recognition
      opsonin binding
      positive regulation of phagocytosis
      sugar binding
      response to molecule of fungal origin
      detection of yeast
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg, 25 µg
      1/5 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/50
      Functional Assays
      Immunohistology - Paraffin
      Immunohistology - Resin
      Immunoprecipitation
      Immunohistology - Frozen(1)
      (1)
      The epitope recognised by 2A11 is sensitive to H202 treatment, therefore quenching with 0.3% H202 should be performed after incubation with the primary antibody. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat1/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg, 25 µg
      1/5 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg, 25 µg
      1/5 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Neat Pack Size: 0.1 mg, 25 µg
      1/5 Pack Size: 0.5 mg
      1/10Pack Size: 0.1 mg, 25 µg
      1/50Pack Size: 0.5 mg

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
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    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional Dectin-1 Antibody Formats

    Formats Clone Applications Sizes available
    Dectin-1 Antibody : Biotin 2A11 F 25 µg | 0.1 mg
    Dectin-1 Antibody : Alexa Fluor® 647 2A11 F 100 Tests/1ml | 25 Tests/0.25ml
    Dectin-1 Antibody : Purified 2A11 C *, F, IP 25 µg | 0.25 mg | 0.1 mg
    Dectin-1 Antibody : Low Endotoxin 2A11 C *, F, FN, IP 0.5 mg
    Dectin-1 Antibody : FITC 2A11 F 25 µg | 50 µg | 0.5 mg | 0.1 mg
    Dectin-1 Antibody : RPE 2A11 F 100 Tests | 25 Tests
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)STAR131A1 mlC, E, P, WB
      STAR131A
      Goat anti Rat IgG:Biotin (Mouse Adsorbed)STAR131B0.5 mgC, E, IF, P, WB
      STAR131B
      Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed)STAR71D549GA0.1 mgF, IF
      STAR71D549GA
      Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed)STAR71D649GA0.1 mgF, IF
      STAR71D649GA
      Rabbit F(ab')2 anti Rat IgG:Dylight®800STAR16D800GA0.1 mgF, IF, WB
      STAR16D800GA
      Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)STAR71D800GA0.1 mgF, IF, WB
      STAR71D800GA
      Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)STAR690.5 mlF
      STAR69
      Rabbit F(ab')2 anti Rat IgG:FITCSTAR17B1 mgF
      STAR17B
      Goat anti Rat IgG:HRP (Mouse Adsorbed)STAR720.5 mgC, E, P
      STAR72
      Rabbit F(ab')2 anti Rat IgG:HRPSTAR21B1 mgC, E, P, RE
      STAR21B
      Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)STAR730.5 mlF
      STAR73
      Rabbit F(ab')2 anti Rat IgG:RPESTAR20A1 mlF
      STAR20A
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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