Clathrin Antibody | BF-06

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  • Mouse anti Clathrin Heavy Chain
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  • Product Type
    Monoclonal Antibody
  • Clone
    BF-06
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2883E, IF, IP, WBdatasheet pdfdatasheet pdf0.1 mg
    MCA2883
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human Clathrin antibody, clone BF-06 recognizes clathrin heavy chain (CHC). Clathrin heavy chain 1 is a 1674 amino acid membrane protein with a predicted molecular mass of ~192 kDa. Clathrin is a ubiquitously expressed protein and major component of clathrin-coated vesicles and pits, which combines with clathrin light chains (CLC) to form clathrin triskelions (3CHC and 3CLC), which then self-assemble into a polyhedral coat. Clathrin-coated vesicles and pits are involved in receptor-mediated endocytosis and secretory vesicle formation, and are linked with the plasma membrane or trans-Golgi network by adaptor proteins.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Humanyes
      Bovineyes
      Pigyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ion exchange chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Recombinant mouse SSRP1, amino acid residues 150-413 (Makurek et al. 2003) .
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      vesicle-mediated transport
      structural molecule activity
      clathrin coat of coated pit
      binding
      intracellular protein transport
      clathrin coat of trans-Golgi network vesicle
      mitochondrion
      melanosome
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunofluorescence
      Immunoprecipitation
      Western Blotting

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      MCA2883 detects a band of approximately 180kDa in mouse and porcine brain cell lysates, and in rat basophilic leukaemia (RBL) and human cervical adenocarcinoma (HeLa) cell lysates.
    • Instructions For Use

    Additional Clathrin Antibody Formats

    Formats Clone Applications Sizes available
    Clathrin Antibody : Purified BF-06 E, IF, IP, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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