Choline Acetyltransferase Antibody

Choline Acetyltransferase Antibody gallery image 1

Western blotting analysis of Mouse Brain lysate (35μgml-1g protein in RIPA buffer) probed using Goat anti Human choline acetyl transferase antibody (AHP1569) at 0.2μgml-1. Primary incubation was 1 hour. Signal was detected by chemiluminescence

Choline Acetyltransferase Antibody gallery image 2

Immunohistochemichal staining of formalin fixed cryosection of mouse basal forebrain using Goat anti choline acetyl transferase (AHP1569) at 0.1μgml-1 followed by HRP-staining with Ni-DAB after Biotin-SP-antigoat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med, Budapest, Hungary

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  • Goat anti Mouse Choline Acetyltransferase (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1569E, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Mouse choline acetyltransferase antibody recognizes an epitope within the C-terminal (CT) region of mouse choline acetyltransferase (Chat), an enzyme which catalyzes the conversion of acetyl CoA and choline into the neurotransmitter acetylcholine (Ach), at both central and peripheral cholinergic synapses.

      Abnormalities in Chat expression are linked with the neurodegenerative disease Alzheimer's, and also with schizophrenia and sudden infant death syndrome.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse chat (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      0.02% Sodium Azide (NaN3)
      0.5% Bovine Serum Albumin
    • Immunogen
      Peptide sequence C-KERARGPTKPSNLD corresponding to the C-Terminal region of choline acetyltransferase (NP_034021.1).
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      rhythmic behavior
      establishment of synaptic specificity at neuromuscular junction
      rhythmic excitation
      neuromuscular synaptic transmission
      neuronal cell body
      choline O-acetyltransferase activity
      adult walking behavior
      dendrite development
      neurotransmitter biosynthetic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.20.5ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1569 detects a band of approximately 75-80kDa in mouse and rat brain cell lysates.
    • Instructions For Use

    Additional Choline Acetyltransferase Antibody Formats

    Formats Applications Sizes available
    Choline Acetyltransferase Antibody : Purified E, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

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