Browsing Modes for Business Users

CDw210a antibody | 1B1.3a

Rat anti Mouse CDw210a

Product Type
Monoclonal Antibody
Clone
1B1.3a
Isotype
IgG1
Specificity
CDw210a

Product Code Applications Pack Size List Price Your Price Qty
MCA2256
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 0.25 mg loader
List Price Your Price
loader

Rat anti Mouse CDw210a antibody, clone 1B1.3a recognizes mouse CDw210a, a cell surface glycoprotein of approximately 110 kDa, also known as Interleukin-10 receptor 1 (IL-10R1). The epitope recognized by this antibody has been localised to the ligand binding domain within the extracellular region of mouse CDw210a. Mouse CDw210a is expressed by a range of cells including thymocytes, T cells, B cells and monocytes. Rat anti Mouse CDw210a antibody, clone 1B1.3a is reported to neutralize the effects of IL-10 (Asseman et al. 1997).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Purified recombinant extracellular region of mouse CDw210a.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Fisher rats were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 1x106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
List Price Your Price
loader
Description Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed)
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Biotin (Mouse Adsorbed)
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:Dylight®800
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:FITC
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
List Price Your Price
loader
Description Rabbit F(ab')2 anti Rat IgG:HRP
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed) STAR71D550 F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) STAR71D650 F IF 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
List Price Your Price
loader
Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader
List Price Your Price
loader
Description Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed)

References for CDw210a antibody

  1. Asseman, C. et al. (1999) An essential role for interleukin 10 in the function of regulatory T cells that inhibit intestinal inflammation.
    J Exp Med. 190 (7): 995-1004.
  2. Phillips, J.M. et al. (2001) Cutting edge: interactions through the IL-10 receptor regulate autoimmune diabetes.
    J Immunol. 167 (11): 6087-91.
  3. Iwashiro, M. et al. (2001) Immunosuppression by CD4+ regulatory T cells induced by chronic retroviral infection.
    Proc Natl Acad Sci U S A. 98: 9226-30.
  4. Montagnoli, C. et al. (2002) B7/CD28-dependent CD4+CD25+ regulatory T cells are essential components of the memory-protective immunity to Candida albicans.
    J Immunol. 169: 6298-308.
  5. Vicari, A.P. et al. (2002) Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody.
    J Exp Med. 196: 541-9.
  6. Sundstedt, A. et al. (2003) Role for IL-10 in suppression mediated by peptide-induced regulatory T cells in vivo.
    J Immunol. 170 (3): 1240-8.
  7. Asseman, C. et al. (2003) Colitogenic Th1 cells are present in the antigen-experienced T cell pool in normal mice: control by CD4+ regulatory T cells and IL-10.
    J Immunol. 171: 971-8.

Functional Assays

Synonyms
IL-10R1
RRID
AB_2125250
UniProt
Q61727
Entrez Gene
Il10ra
GO Terms
GO:0007596 blood coagulation
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA2256

157788 164042 1708

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

Request a different product with this specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products
Please note