Rat anti Mouse CD94 antibody, clone 18d3 recognizes murine CD94, a 70kDa cell surface glycoprotein expressed primarily by mouse natural killer (NK) cells. The murine CD94 molecule forms heterodimers with NKG2A, C or E subunits to form the CD94/NKG2 receptor. In mice the CD94/NKG2 receptor recognizes Qa-1b, a non-classical MHC class 1 molecule. CD94/NKG2 receptors may play an important role in self-tolerance of NK cells during fetal and neonatal life. N.B. Recent reports suggest that DBA/2J mice do not express cell surface CD94/NKG2A receptors (Vance et al. 2002).
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
CHO cells transfected with B6 allele of CD94
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Spleen cells from immunised Lewis rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul