CD90 Antibody | F7D5

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CD90 Antibody | F7D5 gallery image 1

Staining of mouse thymus cells with Mouse anti Mouse CD90 (Thy 1.2) (MCA02R)

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CD90 Antibody | F7D5 gallery image 2

Published customer image:
Mouse anti Mouse CD90 antibody, clone F7D5 used for T cell depletion of bone marrow and subsequent evaluation of chimerism by flow cytometry.
Image caption:
Donor chimerism and alloreactivity. a Percentage donor T cell chimerism in peripheral blood lymphocytes, determined by Thy1.1 (recipient) and Thy1.2 (donor) positivity. b percentage alloreactive CD3+Vß6+ T cells. Results are mean ± SEM from 5 animals in BMT+SPL+SRI and BMT only group and from 4 animals in BMT+SPL+vehicle group. Statistically significant differences are depicted with * (one-tailed p<0.05). NS = not significant

From: Gobin V, Van Steendam K, Fevery S, Tilleman K, Billiau AD, Denys D, Deforce DL. Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression. J Neuroimmune Pharmacol. 2013 Sep;8(4):934-43.

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  • Product Type
    Monoclonal Antibody
  • Clone
    F7D5
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA02RCT, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA02R
    MCA02RTCT, Fdatasheet pdfdatasheet pdf25 µg
    MCA02RT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.

      The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake, P et al. 1979).

      Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/101/25
    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/101/25

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD90 Antibody Formats

    Formats Clone Applications Sizes available
    CD90 Antibody : Purified F7D5 CT, F 25 µg | 0.25 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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