CD90 Antibody | F7D5

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CD90 Antibody | F7D5 gallery image 1

Figure A. RPE conjugated rat anti mouse CD4 (MCA2691PE). Figure B. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A488 conjugated mouse anti mouse CD90 (MCA02A488). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5™ cell analyzer.

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CD90 Antibody | F7D5 gallery image 2

Figure A. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A647 conjugated mouse IgM isotype control (MCA722A647). Figure B. RPE conjugated rat anti mouse CD4 (MCA2691PE) and A647 conjugated mouse anti mouse CD90 (MCA02A647). All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5™ cell analyzer.

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CD90 Antibody | F7D5 gallery image 3

Published customer image:
Mouse anti Mouse CD90 antibody, clone F7D5 used for T cell depletion of bone marrow and subsequent evaluation of chimerism by flow cytometry.
Image caption:
Donor chimerism and alloreactivity. a Percentage donor T cell chimerism in peripheral blood lymphocytes, determined by Thy1.1 (recipient) and Thy1.2 (donor) positivity. b percentage alloreactive CD3+Vß6+ T cells. Results are mean ± SEM from 5 animals in BMT+SPL+SRI and BMT only group and from 4 animals in BMT+SPL+vehicle group. Statistically significant differences are depicted with * (one-tailed p<0.05). NS = not significant

From: Gobin V, Van Steendam K, Fevery S, Tilleman K, Billiau AD, Denys D, Deforce DL. Fluoxetine reduces murine graft-versus-host disease by induction of T cell immunosuppression. J Neuroimmune Pharmacol. 2013 Sep;8(4):934-43.

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  • CD90 Antibody | F7D5 thumbnail image 1
  • CD90 Antibody | F7D5 thumbnail image 2
  • CD90 Antibody | F7D5 thumbnail image 3
  • Mouse anti Mouse CD90:Alexa Fluor® 647
  • Mouse anti Mouse CD90:Pacific Blue®
  • Mouse anti Mouse CD90:RPE
  • Mouse anti Mouse CD90:Alexa Fluor® 488
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    F7D5
  • Isotype
    IgM
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA02RCT, Fdatasheet pdfdatasheet pdf0.25 mg
    MCA02R
    MCA02PEFdatasheet pdfdatasheet pdf100 Tests
    MCA02PE
    MCA02A647Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA02A647
    MCA02PBFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA02PB
    MCA02A488Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA02A488
    MCA02RTCT, Fdatasheet pdfdatasheet pdf25 µg
    MCA02RT
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Mouse CD90 antibody, clone F7D5 recognizes the mouse Thy1.2 alloantigen, also known as CD90.2, which is expressed by thymocytes and peripheral T lymphocytes. Clone F7D5 reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.

      The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity (Lake, P et al. 1979).

      Mouse anti Mouse CD90 antibody, clone F7D5 is routinely tested in flow cytometry using mouse thymocytes.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      IgM fraction conjugated to Alexa Fluor 647 - liquid
      IgM fraction conjugated to Pacific Blue - liquid
      IgM fraction - liquid
      IgM fraction conjugated to R. Phycoerythrin (RPE) - lyophilized
      IgM fraction conjugated to Alexa Fluor 488 - liquid
      IgM fraction - liquid
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
      Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
      IgM fraction prepared by ammonium sulfate precipitation
      IgM fraction prepared by ammonium sulfate precipitation
      IgM fraction prepared by ammonium sulphate precipitation
      IgM fraction prepared by ammonium sulfate precipitation
      IgM fraction prepared by ammonium sulfate precipitation
      IgM fraction prepared by ammonium sulphate precipitation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      5% Sucrose
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      0.09%Sodium Azide
    • Purity
    • Approx. Protein Concentrations
      Ig concentration 0.05 mg/ml
      IgM concentration 0.05 mg/ml
      IgM concentration 1.0 mg/ml
      Ig concentration 0.05 mg/ml
      IgM concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
      18 months from date of despatch.
      12 months from date of reconstitution
      18 months from date of despatch
      18 months from date of despatch.
    • GO Terms
      negative regulation of T cell receptor signaling pathway
      positive regulation of T cell activation
      growth cone
      angiogenesis
      focal adhesion assembly
      positive regulation of release of sequestered calcium ion into cytosol
      negative regulation of axonogenesis
      Rho GTPase activator activity
      cell-cell adhesion
      membrane raft
      retinal cone cell development
      negative regulation of cell migration
      positive regulation of GTPase activity
      negative regulation of protein kinase activity
      T cell receptor signaling pathway
      dendrite
      anchored to external side of plasma membrane
      GPI anchor binding
      cytoskeleton organization
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/101/25
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Cytotoxic Assays
      Flow Cytometry1/101/25

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD90 Antibody Formats

    Formats Clone Applications Sizes available
    CD90 Antibody : Pacific Blue® F7D5 F 100 Tests/1ml
    CD90 Antibody : Purified F7D5 CT, F 25 µg | 0.25 mg
    CD90 Antibody : Alexa Fluor® 488 F7D5 F 100 Tests/1ml
    CD90 Antibody : RPE F7D5 F 100 Tests
    CD90 Antibody : Alexa Fluor® 647 F7D5 F 100 Tests/1ml
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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