Rat anti Mouse CD71 antibody, clone ER-MP21 recognizes murine CD71, a 200kD antigen which is otherwise known as transferrin receptor. CD71 is expressed by all proliferating cell populations and exists as a homodimer at the cell surface.
Clone ER-MP21 inhibits iron uptake and cell proliferation but does not compete with transferrin for binding to its receptor, which suggests that clone ER-MP21 binds to an epitope that is different from the transferrin-binding site (Leenen et al. 1990).
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Balb/c macrophage precursor cell hybrids
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul.