CD54 Antibody | 3E2B

CD54 Antibody | 3E2B gallery image 1

Published customer image:
Hamster anti Mouse CD54 antibody, clone 3E2B used for the detection of Mouse ICAM-1 in murine liver cryosections by immunohistochemistry.
Image caption:
Expression of lymphoid structure markers in livers of aly BM-chimeric mice. Liver cryosections from aly BM-chimeras immunized s.c. with MOG35–55 (d11) were stained with antibodies against CD4, CD8, CD11b, CD11c, CD19, CD62L, CD68, FDC, ICAM, Ki67, PNA, and VCAM. Bar indicates 200 μm.

From: Greter M, Hofmann J, Becher B (2009)
Neo-Lymphoid Aggregates in the Adult Liver Can Initiate Potent Cell-Mediated Immunity.
PLoS Biol 7(5): e1000109.

  • CD54 Antibody | 3E2B thumbnail image 1
  • Hamster anti Mouse CD54:Preservative Free
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1371ZC, E, F, FNdatasheet pdfdatasheet pdf0.5 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Hamster anti Mouse CD54 antibody, clone 3E2B recognizes murine Intercellular adhesion molecule 1, also known as ICAM-1, CD54, MALA-2 or MyD10. CD54 is a 537 amino acid ~89kDa single pass type I transmembrane glycoprotein bearing five Ig-like C2 type domains. Two isoforms, differing at the N-terminal signal peptide sequence and flanking end terminal domain of the processed molecule are generrated by alternative splicing(UniProt P13577) . CD45 is a counter-receptor for LFA-1 (Diamond et al. 1990)

      Murine CD54 is expressed at low levels on T-cells, B-cells, monocytes and endothelial cells but can be induced strongly by a number of stimuli, including UV radiation (Tang and Udey 1991).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      None present.

      Sterile filtered.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      Please see label for expiry date.
    • GO Terms
      regulation of cell adhesion
      leukocyte cell-cell adhesion
      external side of plasma membrane
      immunological synapse
      T cell antigen processing and presentation
      cell adhesion mediated by integrin
      integral to membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat
      Functional Assays
      Immunohistology - Frozen

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD54 Antibody Formats

    Formats Clone Applications Sizes available
    CD54 Antibody : Preservative Free 3E2B C, E, F, FN 0.5 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Immunohistology - Frozen


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