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CD49d antibody | PS/2

Product Code Applications Pack Size List Price Your Price Qty
MCA1230GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader

Rat anti Mouse CD49d monoclonal antibody, clone PS/2 recognizes murine alpha 4 integrin (CD49d), a ~150 kDa single pass type I membrane glycoprotein that can associate with either beta 1 integrin (CD29) or beta 7 integrin to form heterodimers CD49d/CD29 (VLA-4) and alpha4/beta7 (LPAM-1) respectively (Holzmann et al. 1989). CD49d is expressed on most lymphocytes, granulocytes, monocytes and thymocytes. The primary ligands for CD49d are CD106 (VCAM-1), fibronectin and MAdCAM-1 (Sheppard et al. 1994).

Clone PS/2 has also been reported to block the binding of CD49d to its ligands (Andrew et al. 1994).

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
P815 DBA/2 murine mastocytoma cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized Fisher rats were fused with SP2/0 mouse myeloma cells
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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Description Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed)
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Description Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed)
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Description Goat anti Rat IgG:HRP (Mouse Adsorbed)
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References for CD49d antibody

  1. Miyake, K. et al. (1991) Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis.
    J Exp Med. 173 (3): 599-607.
  2. Miyake, K. et al. (1991) A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture.
    J Cell Biol. 114 (3): 557-65.
  3. Andrew, D.P. et al. (1994) Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation.
    J Immunol. 153 (9): 3847-61.
  4. Tchilian, E.Z. et al. (1997) Anti-alpha 4 integrin antibody induces apoptosis in murine thymocytes and staphylococcal enterotoxin B-activated lymph node T cells.
    Immunology. 92: 321-7.
  5. Enghofer, M. et al. (1998) Lymphocyte transfer in streptozotocin-induced diabetes: adhesion of donor cells to islet endothelium.
    Am J Physiol. 274: E928-35.
  6. Liu, Z.J. et al. (1999) A novel role for H-Ras in the regulation of very late antigen-4 integrin and VCAM-1 via c-Myc-dependent and -independent mechanisms.
    J Immunol. 163: 4901-8.
  7. Tanneau, G.M. et al. (1999) Differential recruitment of T- and IgA B-lymphocytes in the developing mammary gland in relation to homing receptors and vascular addressins.
    J Histochem Cytochem. 47: 1581-92.
  8. Fukuoka, M. et al. (2000) Antiadhesive function of 130-kd glycoform of CD43 expressed in CD4 T-lymphocyte clones and transfectant cell lines.
    Blood. 96: 4267-75.
  9. View The Latest Product References
  10. Hokibara, S. et al. (2000) Effects of monoclonal antibodies to adhesion molecules on eosinophilic myocarditis in Toxocara canis-infected CBA/J mice.
    Clin Exp Immunol. 114: 236-44.
  11. Bowden, R.A. et al. (2002) Role of alpha4 integrin and VCAM-1 in CD18-independent neutrophil migration across mouse cardiac endothelium.
    Circ Res. 90: 562-9.
  12. Hirata, T. et al. (2002) P-, E-, and L-selectin mediate migration of activated CD8+ T lymphocytes into inflamed skin.
    J Immunol. 169: 4307-13.
  13. Maus, U.A. et al. (2004) Pneumolysin-induced lung injury is independent of leukocyte trafficking into the alveolar space.
    J Immunol. 173: 1307-12.
  14. Ferrer, P. et al. (2005) Association between pterostilbene and quercetin inhibits metastatic activity of B16 melanoma.
    Neoplasia. 7: 37-47.
  15. Eshghi, S. et al. (2007) Alpha4beta1 integrin and erythropoietin mediate temporally distinct steps in erythropoiesis: integrins in red cell development.
    J Cell Biol. 177: 871-80.
  16. Vaz, R. et al. (2012) Fibronectin promotes migration, alignment and fusion in an in vitro myoblast cell model.
    Cell Tissue Res. 348: 569-78.
  17. Zhang, Y. et al. (2012) Autotaxin through lysophosphatidic acid stimulates polarization, motility, and transendothelial migration of naive T cells.
    J Immunol. 189: 3914-24.
  18. Gillberg, L. et al. (2013) Effective treatment of mouse experimental colitis by alpha 2 integrin antibody: comparison with alpha 4 antibody and conventional therapy.
    Acta Physiol (Oxf). 207: 326-36.
  19. Omenetti, S. et al. (2015) Dysregulated intrahepatic CD4+ T-cell activation drives liver inflammation in ileitis-prone SAMP1/YitFc mice.
    Cell Mol Gastroenterol Hepatol. 1 (4): 406-19.
  20. Chung, K.J. et al. (2017) A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity.
    Nat Immunol. 18 (6): 654-64.
  21. Andrews, S.L. et al. (2023) SVEP1 influences monocyte to macrophage differentiation via integrin α4β1/α9β1 and Rho/Rac signalling.
    Biochim Biophys Acta Mol Cell Res. 1870 (6): 119479.

Flow Cytometry

Functional Assays

Immunohistology - Frozen

Synonyms
Integrin Alpha 4 Chain
VLA-4
RRID
AB_566802
UniProt
Q00651
Entrez Gene
Itga4
GO Terms
GO:0007157 heterophilic cell-cell adhesion
GO:0001968 fibronectin binding
GO:0001974 blood vessel remodeling
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007507 heart development
GO:0009897 external side of plasma membrane
GO:0016477 cell migration
GO:0060324 face development
GO:0060710 chorio-allantoic fusion

MCA1230GA

158694 1608

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