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CD3 antibody | 145-2C11

Product Code Applications Pack Size List Price Your Price Qty
MCA2690T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP WB 25 µg loader
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loader
MCA2690
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP WB 0.25 mg loader
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loader
MCA2690GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IF IP WB 0.1 mg loader
List Price Your Price
loader

Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.

Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
MCA2690T, MCA2690: 0.09% Sodium Azide (NaN3)
MCA2690GA: 0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
Approx. Protein Concentrations
MCA2690T, MCA2690: IgG concentration 1.0 mg/ml
MCA2690GA: IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/200
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®550 STAR104D550 F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®550
Goat F(ab')2 anti Hamster IgG:DyLight®650 STAR104D650 F IF 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:DyLight®650
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA F IF WB 0.1 mg loader
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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
Goat F(ab')2 anti Hamster IgG:FITC STAR104F F 0.4 mg loader
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

Source Reference

  1. Leo, O. et al. (1987) Identification of a monoclonal antibody specific for a murine T3 polypeptide.
    Proc Natl Acad Sci U S A. 84 (5): 1374-8.

References for CD3 antibody

  1. Payer, E. et al. (1991) Circulating CD3+/T cell receptor V γ 3+ fetal murine thymocytes home to the skin and give rise to proliferating dendritic epidermal T cells.
    J Immunol. 146 (8): 2536-43.
  2. Salvadori, S. et al. (1994) Abnormal signal transduction by T cells of mice with parental tumors is not seen in mice bearing IL-2-secreting tumors.
    J Immunol. 153 (11): 5176-82.
  3. Lees, C.W. et al. (2008) Analysis of germline GLI1 variation implicates hedgehog signalling in the regulation of intestinal inflammatory pathways.
    PLoS Med. 5: e239.
  4. McDole JR et al. (2010) Rapid formation of extended processes and engagement of Theiler’s virus-infected neurons by CNS-infiltrating CD8 T cells.
    Am J Pathol. 177 (4): 1823-33.
  5. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
    Pharmacol Res. 102: 298-307.
  6. Klemann, C. et al. (2016) Interleukin 17, Produced by γδδ T Cells, Contributes to Hepatic Inflammation in a Mouse Model of Biliary Atresia and Is Increased in Livers of Patients.
    Gastroenterology. 150 (1): 229-241.e5.
  7. Parang, B. et al. (2016) Myeloid translocation genes differentially regulate colorectal cancer programs.
    Oncogene. 35 (49): 6341-9.
  8. Yu, Y. et al. (2017) Conventional alpha beta (αβ) T cells do not contribute to acute intestinal ischemia-reperfusion injury in mice.
    PLoS One. 12 (7): e0181326.
  9. View The Latest Product References
  10. Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
    J Neuroinflammation. 14 (1): 18.
  11. Perrotta, M. et al. (2018) Deoxycorticosterone acetate-salt hypertension activates placental growth factor in the spleen to couple sympathetic drive and immune system activation.
    Cardiovasc Res. 114 (3): 456-67.
  12. Recasens, M. et al. (2019) Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT.
    Glia. 67 (4): 741-58.
  13. Ohmura, Y. et al. (2021) Natural Killer T Cells Are Involved in Atherosclerotic Plaque Instability in Apolipoprotein-E Knockout Mice.
    Int J Mol Sci. 22(22):12451.
  14. Möhn, N. et al. (2020) Synthetic retinoid AM80 inhibits IL-17 production of gamma delta T cells and ameliorates biliary atresia in mice.
    Liver Int. 40 (12): 3031-41.
  15. Shanaki-Bavarsad, M. et al. (2022) Astrocyte-targeted Overproduction of IL-10 Reduces Neurodegeneration after TBI.
    Exp Neurobiol. 31 (3): 173-195.

Immunofluorescence

RRID
AB_905951
UniProt
P22646
Entrez Gene
Cd3e
GO Terms
GO:0001772 immunological synapse
GO:0002669 positive regulation of T cell anergy
GO:0016021 integral to membrane
GO:0004888 transmembrane receptor activity
GO:0008624 induction of apoptosis by extracellular signals
GO:0009897 external side of plasma membrane
GO:0032729 positive regulation of interferon-gamma production
GO:0032753 positive regulation of interleukin-4 production
GO:0042105 alpha-beta T cell receptor complex
GO:0045060 negative thymic T cell selection
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045879 negative regulation of smoothened signaling pathway
GO:0046641 positive regulation of alpha-beta T cell proliferation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050850 positive regulation of calcium-mediated signaling
GO:0050852 T cell receptor signaling pathway

MCA2690T

1612 162077

MCA2690

148852 162079

MCA2690GA

157941 166781 1804

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