CD3 Antibody | 145-2C11

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CD3 Antibody | 145-2C11 gallery image 1

Figure A. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster IgG isotype control (MCA2356A488). Figure B. RPE conjugated Rat anti Mouse CD22 (MCA2187PE) and A488 conjugated Hamster anti Mouse CD3 (MCA2690A488). All experiments were performed on mouse splenocytes in the presence of murine SeroBlock (BUF041A)

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CD3 Antibody | 145-2C11 gallery image 2

Published customer image:
Hamster anti Mouse CD3 antibody, clone 145-2C11 (MCA2690) used for the identification of murine colonic lymphocytes by immunofluorescence.
Image caption:
Intestinal Myeloid Cells Respond Directly to Hh Signalling during Homeostasis and Inflammation (A–C) No DSS treatment. (D–F) 4 d of 3% DSS. (A, D) Few CD3+ T lymphocytes respond to Hh signalling during colonic homeostasis. Infiltrating T cells in DSS-induced ulcers do not express Gli1 or respond to Hh signalling. (B, E) Many but not all CD11b+ myeloid cells respond to Hh signalling and express LacZ. After 4 d of DSS, ulcers in Gli1+/lacZ animals are filled with myeloid cells, many of which respond to Hh signals. (C, F) CD11c+ dendritic cells respond directly to Hh signals during homeostasis and inflammation. Nuclear DAPI staining was performed but has been omitted to allow clear visualization of co-expression of nuclear LacZ and cell surface markers.

From: Lees CW, Zacharias WJ, Tremelling M, Noble CL, Nimmo ER, Tenesa A, et al. (2008) Analysis of Germline GLI1 Variation Implicates Hedgehog Signalling in the Regulation of Intestinal Inflammatory Pathways.
PLoS Med 5(12): e239.

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  • CD3 Antibody | 145-2C11 thumbnail image 1
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  • Hamster anti Mouse CD3
  • Hamster anti Mouse CD3:Alexa Fluor®488
  • Hamster anti Mouse CD3
  • Hamster anti Mouse CD3
  • Hamster anti Mouse CD3:Alexa Fluor®488
  • Hamster anti Mouse CD3:Biotin
(Rated 0.0 out of 5 based on 0 customer reviews)
    CD3 epsilon antibody, clone 145-2C11, is a 20kDa transmembrane protein on T cells, NKT cells and present at different levels of T cell differentiation. It's useful for in vitro blocking, activation assays, apoptosis induction and in vitro T cell depletions.
    • Product Type
      Monoclonal Antibody
    • Clone
      145-2C11
    • Isotype
      IgG
    3 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      MCA2690GAC, F, IF, IP, WBdatasheet pdfdatasheet pdf0.1 mg
      MCA2690GA
      MCA2690BFdatasheet pdfdatasheet pdf0.1 mg
      MCA2690B
      MCA2690C, F, IF, IP, WBdatasheet pdfdatasheet pdf0.25 mg
      MCA2690
      MCA2690A488TFdatasheet pdfdatasheet pdf0.25 ml
      MCA2690A488T
      MCA2690A488Fdatasheet pdfdatasheet pdf1 ml
      MCA2690A488
      MCA2690TC, F, IF, IP, WBdatasheet pdfdatasheet pdf25 µg
      MCA2690T
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Hamster anti Mouse CD3 antibody, clone 145-2C11 detects CD3 epsilon (CD3ε), a ~20 kDa transmembrane protein also known as CD3 or T3. CD3ε is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, and these are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

        CD3ε is primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.

        Hamster anti Mouse CD3 antibody, clone 145-2C11 is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vitro T cell depletions.
      • Intended Use
      • Target Species
        Mouse
      • Product Form
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor 488 - liquid
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG conjugated to Alexa Fluor 488 - liquid
        Purified IgG conjugated to Biotin - liquid
      • Reconstitution
      • Preparation
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
        Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      • Preservative Stabilisers
        Pack Size: 0.25 mg, 25 µg
        0.09%Sodium Azide (NaN3)
        Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        Pack Size: 0.25 mg, 25 µg
        0.09%Sodium Azide (NaN3)
        Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
        Pack Size: 0.25 mg, 25 µg
        0.09%Sodium Azide (NaN3)
        Pack Size: 0.1 mg0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        0.09%Sodium Azide (NaN3)
      • Immunogen
        H-2Kb - specific mouse cytotoxic T lymphocyte clone BM10-37.
      • Purity
      • Approx. Protein Concentrations
        Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgG concentration 1.0mg/ml
        IgG concentration 0.05mg/ml
        Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgG concentration 1.0mg/ml
        Pack Size: 0.25 mg, 25 µgIgG concentration 1.0 mg/ml
        Pack Size: 0.1 mgIgG concentration 1.0mg/ml
        IgG concentration 0.05mg/ml
        IgG concentration 0.5mg/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Fusion Partners
        Spleen cells from hyperimmunized Armenian hamsters (Cricetulus migratorius) were fused with cells of the murine SP2/0 myeloma.
      • Storage
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
        Store at +4oC or at -20oC if preferred.
        Storage in frost-free freezers is not recommended.
        This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      • Shelf Life
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
        18 months from date of despatch.
      • GO Terms
        T cell receptor signaling pathway
        external side of plasma membrane
        positive regulation of T cell anergy
        negative thymic T cell selection
        positive regulation of calcium-mediated signaling
        immunological synapse
        induction of apoptosis by extracellular signals
        negative regulation of smoothened signaling pathway
        positive regulation of alpha-beta T cell proliferation
        positive regulation of interferon-gamma production
        alpha-beta T cell receptor complex
        transmembrane receptor activity
        positive regulation of interleukin-4 production
        positive regulation of interleukin-2 biosynthetic process
        positive regulation of peptidyl-tyrosine phosphorylation
        integral to membrane
      • UniProt
      • Entrez Gene
      • Acknowledgements
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
        This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/251/200
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Western Blotting
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/251/200
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Western Blotting
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry1/251/200
        Immunofluorescence
        Immunohistology - Frozen
        Immunoprecipitation
        Western Blotting
      • Application NameYesNoMin DilutionMax Dilution
        Flow CytometryNeat
      • Application NameYesNoMin DilutionMax Dilution
        Flow Cytometry

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Technical Advice
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Recommended Protocol
      • Flow Cytometry
        Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

        The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • ELISA
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Immunohistology
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Immunofluorescence
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Western Blotting
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use
      • Instructions For Use

      Additional CD3 Antibody Formats

      Formats Clone Applications Sizes available
      CD3 Antibody : Purified 145-2C11 C, F, IF, IP, WB 25 µg | 0.25 mg | 0.1 mg
      CD3 Antibody : Biotin 145-2C11 F 0.1 mg
      CD3 Antibody : Alexa Fluor® 488 145-2C11 F 0.25 ml | 1 ml
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
        STAR79B
        Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
        STAR104D549GA
        Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
        STAR104D649GA
        Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
        STAR104D800GA
        Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
        STAR104F
        Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
        STAR79F
        Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
        STAR79P
        Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
        STAR79PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
        STAR79B
        Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
        STAR104D549GA
        Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
        STAR104D649GA
        Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
        STAR104D800GA
        Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
        STAR104F
        Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
        STAR79F
        Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
        STAR79P
        Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
        STAR79PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
        STAR79B
        Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
        STAR104D549GA
        Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
        STAR104D649GA
        Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
        STAR104D800GA
        Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
        STAR104F
        Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
        STAR79F
        Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
        STAR79P
        Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
        STAR79PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Streptavidin:APCSTAR119100 TestsF
        STAR119

        Recommended Negative Isotype Control

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
          MCA2356
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488MCA2356A488100 Tests/1mlF
          MCA2356A488
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
          MCA2356
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
          MCA2356
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Hamster (Armenian) IgG Negative Control:Alexa Fluor® 488MCA2356A488100 Tests/1mlF
          MCA2356A488

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B
            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Seroblock FcRBUF041A0.1 mgF
            BUF041A
            Mouse Seroblock FcRBUF041B0.5 mgF
            BUF041B

            Recommended Positive Controls

              Histology Controls

                • Application NameReference Images
                  Immunofluorescence
                  Immunohistology - Frozen

                References

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