CD28 Antibody | 37.51.1

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Staining of mouse spleen cells with Hamster anti Mouse CD28: Alexa Fluor® 488 (MCA1363A488)

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Staining of mouse spleen cells with Hamster anti Mouse CD28: Alexa Fluor®? 647 (MCA1363A647)

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Staining of mouse spleen cells with Hamster anti Mouse CD28: Biotin (MCA1363B)

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Staining of mouse spleen cells with Hamster anti Mouse CD28:FITC (MCA1363F)

CD28 Antibody | 37.51.1 gallery image 5

Staining of mouse spleen cells with Hamster anti Mouse CD28 (MCA1363)

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  • Hamster anti Mouse CD28
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA1363F, IPdatasheet pdfdatasheet pdf0.2 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Hamster anti Mouse CD28 antibody, clone 37.51.1 recognizes murine CD28, a ~80kDa cell surface glycoprotein that is expressed by most thymocyte subsets and by peripheral T-cells. Studies show that CD28 expression on thymocytes and splenic T-cells can be upregulated upon activation.
      Murine CD28 acts as a co-stimulatory signal and may play an important role in regulating the immune response of T-cells.
      Hamster anti Mouse CD28 antibody, clone 37.51.1 has been reported to enhance cytokine production and T-cell proliferation in combination with TCR-mediated signals (Gross et al. 1992).
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Mouse T-Lymphoma EL-4 cells.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Borate buffered saline.
    • Fusion Partners
      Spleen cells from immunised Syrian Hamsters were fused with cells of the mouse P3XAg8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      immune response
      negative thymic T cell selection
      integral to membrane
      positive regulation of alpha-beta T cell proliferation
      positive regulation of isotype switching to IgG isotypes
      positive regulation of mitosis
      positive regulation of interleukin-2 biosynthetic process
      positive regulation of inflammatory response to antigenic stimulus
      external side of plasma membrane
      T cell costimulation
      induction of apoptosis by extracellular signals
      positive regulation of transcription from RNA polymerase II promoter
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNEAT

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 5ul of the suggested working dilution to label 106 cells in 100ul.

      The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR BUF041A/BUF041B.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD28 Antibody Formats

    Formats Clone Applications Sizes available
    CD28 Antibody : Purified 37.51.1 F, IP 0.2 mg
    • Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF

        Useful Reagents

          Recommended Positive Controls

            Histology Controls


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