Hamster anti Mouse CD278, clone C398.4A recognizes CD278 also known as ICOS (inducible T cell co-stimulatory molecule) or H4. CD278 is a member of the CD28 family (Hutloff et al. 1999) which also contains the immune attenuator CTLA-4. CD278 expression is found following T cell activation (Yoshinaga et al. 1999) and is able to co-stimulate T cell proliferative responses. ICOS binds to ICOS ligand which is expressed on antigen presenting cells. This ICOS/ICOSL interaction activates PI3K/AKT, MAPK and NKFB signalling pathways in a manner distinct from that acitvated via CD28. In a larger focus ICOS signaling plays a role in several physioogical processes such as T follicular helper cell induction and maturation, antibody affininty maturation and regulation of T helper cell inflammatory responses.
N.B. Antibody reactivity and working conditions may vary between species.
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Purified IgG prepared by affinity chromatography
0.09% Sodium Azide (NaN3)
Mouse T cell clone D10.G4.1
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul