CD278 Antibody | C398.4A

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  • Hamster anti Mouse CD278
  • Hamster anti Mouse CD278:FITC
  • Hamster anti Mouse CD278:RPE
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    C398.4A
  • Isotype
    IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA6051GAFdatasheet pdfdatasheet pdf0.1 mg
    MCA6051GA
    MCA6051FFdatasheet pdfdatasheet pdf0.1 mg
    MCA6051F
    MCA6051PEFdatasheet pdfdatasheet pdf100 Tests/0.5ml
    MCA6051PE
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse CD278, clone C398.4A recognizes CD278 also known as ICOS (inducible T cell co-stimulatory molecule) or H4. CD278 is a member of the CD28 family (Hutloff et al. 1999) which also contains the immune attenuator CTLA-4. CD278 expression is found following T cell activation (Yoshinaga et al. 1999) and is able to co-stimulate T cell proliferative responses. ICOS binds to ICOS ligand which is expressed on antigen presenting cells. This ICOS/ICOSL interaction activates PI3K/AKT, MAPK and NKFB signalling pathways in a manner distinct from that acitvated via CD28. In a larger focus ICOS signaling plays a role in several physiological processes such as T follicular helper cell induction and maturation, antibody affininty maturation and regulation of T helper cell inflammatory responses.
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Ratyes
      Humanyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Mouse T cell clone D10.G4.1
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch
      18 months from date of despatch
      18 months from date of despatch
    • GO Terms
      protein binding
      external side of plasma membrane
      integral to plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/21/10

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD278 Antibody Formats

    Formats Clone Applications Sizes available
    CD278 Antibody : FITC C398.4A F 0.1 mg
    CD278 Antibody : RPE C398.4A F 100 Tests/0.5ml
    CD278 Antibody : Purified C398.4A F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Further Reading

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