CD27 Antibody | LG.3A10

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CD27 Antibody | LG.3A10 gallery image 1

Figure A. APC conjugated Mouse anti Human CD19 (MCA1940APC) and FITC conjugated Armenian Hamster IgG isotype control (MCA2356F). Figure B. APC conjugated Mouse anti Human CD19 (MCA1940APC) and FITC conjugated Armenian Hamster anti Mouse CD27 (MCA4701F). All experiments performed on human peripheral blood lymphocytes in the presence of human SeroBlock (BUF070A).

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  • CD27 Antibody | LG.3A10 thumbnail image 1
  • Hamster anti Mouse CD27:FITC
  • Hamster anti Mouse CD27:RPE
  • Hamster anti Mouse CD27:Biotin
  • Hamster anti Mouse CD27
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    LG.3A10
  • Isotype
    IgG
4 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4701FFdatasheet pdfdatasheet pdf0.1 mg
    MCA4701F
    MCA4701PEFdatasheet pdfdatasheet pdf0.1 mg
    MCA4701PE
    MCA4701BC, F, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA4701B
    MCA4701C, F, IPdatasheet pdfdatasheet pdf0.25 mg
    MCA4701
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse CD27 antibody, clone LG.3A10 recogniszes mouse, rat and human CD27, a member of the tumour necrosis factor receptor superfamily. It is expressed in the thymus and spleen, but not in non-lymphoid tissues. CD27 acts as a ligand for CD70, which is expressed in T and B cells, and acts as a costimulatory signal in lymphocyte proliferation and immunoglobulin production.

      CD27 lacks a death domain in its short cytoplasmic tail, but can induce apoptosis by binding with the apoptosis regulatory protein Siva (also known as CD27-binding protein).
    • Intended Use
    • Target Species
      Mouse
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Humanyes
      Ratyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Armenian hamster fibroblast line ARHO12 transfected with mouse CD27 cDNA.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5mg/ml
      IgG concentration 0.2mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      positive regulation of interferon-gamma biosynthetic process
      induction of apoptosis
      immunoglobulin mediated immune response
      receptor activity
      integral to membrane
      cell surface receptor linked signaling pathway
      positive regulation of T cell differentiation
      integral to plasma membrane
      extracellular region
      release of cytoplasmic sequestered NF-kappaB
      cell surface
      transmembrane receptor activity
      immunoglobulin secretion
      anti-apoptosis
      positive regulation of B cell differentiation
      protein binding
      positive regulation of JNK cascade
      apoptosis
      caspase inhibitor activity
      B cell proliferation
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/50
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/20
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/201/50
      Immunohistology - Frozen
      Immunoprecipitation
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD27 Antibody Formats

    Formats Clone Applications Sizes available
    CD27 Antibody : Purified LG.3A10 C, F, IP 0.25 mg
    CD27 Antibody : RPE LG.3A10 F 0.1 mg
    CD27 Antibody : Biotin LG.3A10 C, F, IP 0.1 mg
    CD27 Antibody : FITC LG.3A10 F 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
      Goat F(ab')2 anti Hamster IgG:DyLight®549STAR104D549GA0.1 mgF, IF
      STAR104D549GA
      Goat F(ab')2 anti Hamster IgG:DyLight®649STAR104D649GA0.1 mgF, IF
      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          BUF041A
          Mouse Seroblock FcRBUF041B0.5 mgF
          BUF041B

          Recommended Positive Controls

            Histology Controls

              References

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