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CD192 Antibody

CD192 Antibody gallery image 1

Paraffin embedded mouse spleen stained with Goat anti mouse CD192 (AAM72)

  • CD192 Antibody thumbnail image 1
  • Goat anti Mouse CD192 (N-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM72F, P *, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Mouse CD192 (N-terminal) antibody recognizes an epitope within the N-terminal (NT) region of mouse CD192, otherwise known as CCR2 (C-C chemokine receptor type 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which plays a key role in inflammation.

      CD192 acts as a receptor for the chemokines MCP-1 (monocyte chemoattractant protein 1), MCP-3 and MCP-4, and interaction between CD192 and MCP-1, initiates chemotactic responses through an increase in intracellular calcium levels.

      Goat anti Mouse CD192 is reported as suitable for use in immunocytochemistry on acetone fixed cells.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse CD192 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide SHSLFTRSIQELDEGATTPYDYDDGEPC corresponding to amino acids 18-45 within the N-terminal region of mouse CD192.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      cellular defense response
      positive regulation of immune complex clearance by monocytes and macrophages
      humoral immune response
      plasma membrane
      C-C chemokine receptor activity
      sensory perception of pain
      integral to membrane
      negative regulation of angiogenesis
      regulation of vascular endothelial growth factor production
      positive regulation of macrophage chemotaxis
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/20
      Western Blotting1/500
      Immunohistology - Paraffin(1)1/125
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD192 Antibody Formats

    Formats Applications Sizes available
    CD192 Antibody : Purified F, P *, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD192 Antibody thumbnail image 1
              • Application NameReference Images
                Flow Cytometry


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