Goat anti Mouse CD192 (N-terminal) antibody recognizes an epitope within the N-terminal (NT) region of mouse CD192, otherwise known as CCR2 (C-C chemokine receptor type 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, which plays a key role in inflammation.
CD192 acts as a receptor for the chemokines MCP-1 (monocyte chemoattractant protein 1), MCP-3 and MCP-4, and interaction between CD192 and MCP-1, initiates chemotactic responses through an increase in intracellular calcium levels.
Goat anti Mouse CD192 is reported as suitable for use in immunocytochemistry on acetone fixed cells.
Purified IgG - liquid
Antiserum to mouse CD192 (NT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
0.1% Sodium Azide (NaN3) 0.1% Bovine Serum Albumin
Synthetic peptide SHSLFTRSIQELDEGATTPYDYDDGEPC corresponding to amino acids 18-45 within the N-terminal region of mouse CD192.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Paraffin(1)
This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.