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CD14 Antibody | biG53

CD14 Antibody | biG53 gallery image 1

Figure A. FITC conjugated rat anti mouse F4/80 (MCA497F). Figure B. FITC conjugated rat anti mouse F4/80 (MCA497F) and RPE conjugated mouse anti mouse CD14 (MCA2745PE). All experiments performed on murine peritoneal macrophages in the presence of murine SeroBlock (BUF041A).

  • CD14 Antibody | biG53 thumbnail image 1
  • Mouse anti Mouse CD14:RPE
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  • Product Type
    Monoclonal Antibody
  • Clone
  • Isotype
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2745PEFdatasheet pdfdatasheet pdf100 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Mouse anti Mouse CD14 antibody, clone biG53 recognizes mouse CD14, a ~55 kDa a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on peripheral blood monocytes, weakly expressed on granulocytes and absent on myeloid progenitors. The CD14 molecule acts as a pattern recognition receptor, which binds lipopolysaccharide (LPS) and other microbial products such as lipoteichoic acid, lipoproteins and peptidoglycan. Binding of LPS to CD14 mediates the innate immune response to LPS and requires the Toll-like receptor-4 (TLR4)/MD-2 complex for effective signalling.

      Mouse anti Mouse CD14 antibody, clone biG53 inhibits the binding of CD14.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
    • Reconstitution
      Reconstitute with 1ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Recombinant mouse CD14.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      innate immune response
      anchored to membrane
      inflammatory response
      membrane raft
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometryneat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD14 Antibody Formats

    Formats Clone Applications Sizes available
    CD14 Antibody : RPE biG53 F 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mouse Seroblock FcRBUF041A0.1 mgF
          Mouse Seroblock FcRBUF041B0.5 mgF

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • CD14 Antibody | biG53 thumbnail image 1

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