Figure A. FITC conjugated rat anti mouse F4/80 (MCA497F). Figure B. FITC conjugated rat anti mouse F4/80 (MCA497F) and RPE conjugated mouse anti mouse CD14 (MCA2745PE). All experiments performed on murine peritoneal macrophages in the presence of murine SeroBlock (BUF041A).
Mouse anti Mouse CD14 antibody, clone biG53 recognizes mouse CD14, a ~55 kDa a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on peripheral blood monocytes, weakly expressed on granulocytes and absent on myeloid progenitors. The CD14 molecule acts as a pattern recognition receptor, which binds lipopolysaccharide (LPS) and other microbial products such as lipoteichoic acid, lipoproteins and peptidoglycan. Binding of LPS to CD14 mediates the innate immune response to LPS and requires the Toll-like receptor-4 (TLR4)/MD-2 complex for effective signalling.
Mouse anti Mouse CD14 antibody, clone biG53 inhibits the binding of CD14.
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitute with 1ml distilled water
Purified IgG prepared by affinity chromatography on Protein G
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin
Recombinant mouse CD14.
Approx. Protein Concentrations
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.