CD103 Antibody | 2E7

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  • Hamster anti Mouse CD103:RPE
  • Hamster anti Mouse CD103:FITC
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    2E7
  • Isotype
    IgG
3 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA4705PEFdatasheet pdfdatasheet pdf0.1 mg
    MCA4705PE
    MCA4705FFdatasheet pdfdatasheet pdf0.1 mg
    MCA4705F
    MCA4705GAC, F, IF, IPdatasheet pdfdatasheet pdf0.1 mg
    MCA4705GA
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Hamster anti Mouse CD103 antibody, clone 2E7 recognizes mouse CD103, otherwise known as integrin alpha-E. CD103 binds Integrin beta 7 to form the heterodimeric integrin molecule αEβ7. This complex is sometimes referred to as CD103, though strictly speaking CD103 only refers to the alpha chain. The beta 7 subunit can also interact with other integrin alpha chains. The αEβ7 complex is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocyes to epithelial cell monolayers. CD103 is expressed on intestinal intraepithelial lymphocytes and is highly expressed on regulatory T cells. Hamster anti Mouse CD103 antibody, clone 2E7 is expected to also recognize the intact αEβ7 complex.
    • Intended Use
    • Target Species
      Mouse
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
      Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Mouse intestinal intraepithelial lymphocytes
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.2 mg/ml
      IgG concentration 0.5 mg/ml
      IgG concentration 0.5mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
      12 months from date of despatch
      18 months from date of despatch.
    • GO Terms
      external side of plasma membrane
      integrin complex
      receptor activity
      cell adhesion
      integrin-mediated signaling pathway
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/51/10
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/5
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/101/20
      Immunofluorescence
      Immunohistology - Frozen
      Immunoprecipitation

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional CD103 Antibody Formats

    Formats Clone Applications Sizes available
    CD103 Antibody : RPE 2E7 F 0.1 mg
    CD103 Antibody : Purified 2E7 C, F, IF, IP 0.1 mg
    CD103 Antibody : FITC 2E7 F 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Hamster IgG:BiotinSTAR79B0.8 mgE, F
      STAR79B
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      STAR104D549GA
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      STAR104D649GA
      Goat F(ab')2 anti Hamster IgG:Dylight®800STAR104D800GA0.1 mgF, IF, WB
      STAR104D800GA
      Goat F(ab')2 anti Hamster IgG:FITCSTAR104F0.4 mgF
      STAR104F
      Goat anti Hamster IgG:FITCSTAR79F0.7 mgF
      STAR79F
      Goat anti Hamster IgG:HRPSTAR79P0.8 mgE
      STAR79P
      Goat anti Hamster IgG:RPESTAR79PE0.1 mgF
      STAR79PE

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:RPEMCA2356PE0.1 mgF
        MCA2356PE
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative Control:FITCMCA2356F0.1 mgF
        MCA2356F
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Hamster (Armenian) IgG Negative ControlMCA23560.25 mgF
        MCA2356

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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