Hamster anti Mouse CD103 antibody, clone 2E7 recognizes mouse CD103, otherwise known as Integrin alpha-E. CD103 binds Integrin beta 7 to form the heterodimeric integrin molecule αEβ7. This complex is sometimes referred to as CD103, though strictly speaking CD103 only refers to the alpha chain. The beta 7 subunit can also interact with other integrin alpha chains. The αEβ7 complex is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. CD103 is expressed on intestinal intraepithelial lymphocytes and is highly expressed on regulatory T cells.Hamster anti Mouse CD103 antibody, clone 2E7 is expected to also recognize the intact αEβ7 complex.
Purified IgG - liquid
Purified IgG prepared by affinity chromatography
0.09% Sodium Azide (NaN3)
Mouse intestinal intraepithelial lymphocytes.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.