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CCR10 Antibody

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Staining of paraffin embedded mouse small intestine with Goat anti mouse CCR10 (AAM69)

  • CCR10 Antibody thumbnail image 1
  • Goat anti Mouse CCR10
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAM69E, F, P, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Goat anti Mouse CCR10 recognizes mouse CCR10 (C-C chemokine receptor type 10), otherwise known as GPR2, (G-protein coupled receptor 2), a transmembrane glycoprotein and member of the G-protein coupled receptor 1 family, highly expressed in the small intestine, colon, lymph nodes and Peyer patches, which acts as a receptor for the chemokines CCL27 (SCYA27/CTACK) and CCL28 (SCYA28).

      This product is reported as suitable for use in immunocytochemistry and shows minimal cross-reactivity with human CCR10.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to mouse CCR10 was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      0.1% Bovine Serum Albumin
    • Immunogen
      Synthetic peptide KPTEQVSWGLYSGYDEEAYSVGPLPELC corresponding to amino acids 4-31 of mouse CCR10.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC only.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      plasma membrane
      elevation of cytosolic calcium ion concentration
      C-C chemokine receptor activity
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Paraffin1/250
      Western Blotting1/1000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
    • ELISA
      This product is suitable for use in indirect ELISA applications.
    • Immunohistology
    • Histology Positive Control Tissue
      Mouse small intestine
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CCR10 Antibody Formats

    Formats Applications Sizes available
    CCR10 Antibody : Purified E, F, P, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
      Rabbit anti Goat IgG (Fc):HRPSTAR122P1 mgC, E, WB

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Mouse small intestine
                • Product Images

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