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ABCA1 Antibody | 3A1-891.3
Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA (MCA2878)
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Staining of pCPT-cAMP stimulated RAW264.7 cells with Rat anti Mouse ABCA1:FITC (MCA2878F)
Enlarge- Rat anti Mouse ABCA1:FITC
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Product TypeMonoclonal Antibody
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Clone3A1-891.3
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IsotypeIgG1
1 Formats Available
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
Summary
Secondary Antibodies
Negative Isotype Controls
Useful Reagents
Positive Controls
Histology Controls
More Images
References
Reviews
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- Rat anti Mouse ABCA1 antibody, clone 3A1-891.3 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.
Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.
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Intended Use
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Target SpeciesMouse
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Product FormPurified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
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Reconstitution
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PreparationPurified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
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Preservative Stabilisers0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin -
ImmunogenSynthetic peptide corresponding to aa 215-233
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Purity
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Approx. Protein ConcentrationsIgG concentration 0.1mg/ml
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Reagents In The Kit
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Preparing The Antibody
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Test Principle
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Buffer SolutionPhosphate buffered saline
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StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. -
Shelf Life18 months from date of despatch.
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GO Termscholesterol effluxcellular response to retinoic acidcholesterol transporter activityGolgi apparatusATP bindingphospholipid effluxphagocytosis, engulfmentanion transmembrane transporter activityprotein lipidationintegral to plasma membranecholesterol metabolic processpositive regulation of cholesterol effluxcellular response to lipopolysaccharidepeptide secretionphospholipid transporter activityphospholipid translocationATPase activityreverse cholesterol transport
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UniProt
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Entrez Gene
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Acknowledgements
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RegulatoryFor research purposes only
- This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Yes No Min Dilution Max Dilution Flow Cytometry Neat 1/10 - Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended Protocol
- Flow CytometryUse 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- ELISA
- Immunohistology
- Histology Positive Control Tissue
- Immunofluorescence
- Western Blotting
- Instructions For Use
Additional ABCA1 Antibody Formats
| Formats | Clone | Applications | Sizes available |
|---|---|---|---|
| ABCA1 Antibody : FITC | 3A1-891.3 | F | 0.1 mg |
- Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)
Recommended Negative Isotype Control
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG1 Negative Control:FITC | MCA1211F | 100 Tests | F | MCA1211F |
Useful Reagents
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse Seroblock FcR | BUF041A | 0.1 mg | F | BUF041A | |
| Mouse Seroblock FcR | BUF041B | 0.5 mg | F | BUF041B |
Recommended Positive Controls
Histology Controls
References
1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.Pflugers Arch. 453 (5): 569-79.
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Formerly known as AbD Serotec, a global supplier and manufacturer of antibodies, kits, and reagents.
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
Copyright © 2018 Bio-Rad Laboratories, Inc. All rights reserved
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