WNV antibody | AbD05320

Human anti WNV NS2B-NS3
- Product Type
- Monoclonal Antibody
- Clone
- AbD05320
- Isotype
- HuCAL Fab bivalent
- Specificity
- WNV
- Region
- NS2B-NS3
Human anti WNV NS2B-NS3 antibody, clone AbD05320 recognizes the NS2B-NS3 proteinase from West Nile virus (WNV). West Nile virus is a member of the family Flaviviridae which in humans is transmitted via a mosquito vector resulting in central nervous damage. Non structural protein 3 (NS3) is a multifunctional protein, which is implicated in polyprotein processing and RNA replication. NS3 requires the presence of non-structural protein 2B (NS2B) cofactor to exhibit its proteolytic activity. |
- Target Species
- Viral
- Product Form
- A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL GOLD phage display library, expressed in E. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
- Preparation
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- NS2B-NS3 proteinase from WNV expressed in E.coli
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | ![]() |
1/1000 | 1/10000 |
Western Blotting | ![]() |
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
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Mouse anti Penta Histidine Tag:HRP | MCA5995P | E WB | 125 µl |
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Mouse anti Histidine Tag:Alk.Phos. | MCA1396A | E WB | 0.1 mg |
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Description | Mouse anti Histidine Tag:Alk.Phos. | ||||||
Mouse anti c-Myc:Biotin | MCA2200B | C E P WB* | 0.1 mg |
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Description | Mouse anti c-Myc:Biotin | ||||||
Mouse anti c-Myc:HRP | MCA2200P | C E P WB* | 0.1 mg |
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List Price | Your Price | ||||||
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Description | Mouse anti c-Myc:HRP | ||||||
Mouse anti Histidine Tag:Biotin | MCA1396B | E WB | 0.1 mg |
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Description | Mouse anti Histidine Tag:Biotin |
References for WNV antibody
-
Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283 (25): 17270-8. -
Shiryaev, S.A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427 (3): 369-76.
- RRID
- AB_1605523
- UniProt
- P06935
- Entrez Gene
- WNVgp1
- GO Terms
- GO:0005515 protein binding
- GO:0003724 RNA helicase activity
- GO:0003725 double-stranded RNA binding
- GO:0004252 serine-type endopeptidase activity
- GO:0003968 RNA-directed RNA polymerase activity
- GO:0016021 integral to membrane
- GO:0004482 mRNA (guanine-N7-)-methyltransferase activity
- GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
- GO:0005198 structural molecule activity
- View More GO Terms
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006410 transcription, RNA-dependent
- GO:0019079 viral genome replication
- GO:0019028 viral capsid
- GO:0019031 viral envelope
- GO:0030683 evasion by virus of host immune response
- GO:0042025 host cell nucleus
- GO:0044167 host cell endoplasmic reticulum membrane
- GO:0045449 regulation of transcription
- GO:0055036 virion membrane
- Licensed Use
- For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
HCA085

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