WNV antibody | AbD05320

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Human anti WNV NS2B-NS3

Product Type: Monoclonal Antibody
Clone: AbD05320
Isotype: HuCAL Fab bivalent

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
HCA085 Datasheet	E WB SDS	0.1 mg	Log in		Get Quote

Human anti WNV NS2B-NS3 antibody, clone AbD05320 recognizes the NS2B-NS3 proteinase from West Nile virus (WNV). West Nile virus is a member of the family Flaviviridae which in humans is transmitted via a mosquito vector resulting in central nervous damage. Non structural protein 3 (NS3) is a multifunctional protein, which is implicated in polyprotein processing and RNA replication. NS3 requires the presence of non-structural protein 2B (NS2B) cofactor to exhibit its proteolytic activity.

Product Details

Target Species: Viral
Product Form: A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL® GOLD phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation: Metal chelate affinity chromatography
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.01% Thiomersal
Immunogen: NS2B-NS3 proteinase from WNV expressed in E.coli
Approx. Protein Concentrations: Total protein concentration 0.5 mg/ml

Storage Information

Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee: 12 months from date of despatch

More Information

UniProt: P06935
Entrez Gene: WNVgp1
GO Terms: GO:0005515 protein binding; GO:0003724 RNA helicase activity; GO:0003725 double-stranded RNA binding; GO:0004252 serine-type endopeptidase activity; GO:0003968 RNA-directed RNA polymerase activity; GO:0016021 integral to membrane; GO:0004482 mRNA (guanine-N7-)-methyltransferase activity; GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity; GO:0005198 structural molecule activity; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0006410 transcription, RNA-dependent; GO:0019079 viral genome replication; GO:0019028 viral capsid; GO:0019031 viral envelope; GO:0030683 evasion by virus of host immune response; GO:0042025 host cell nucleus; GO:0044167 host cell endoplasmic reticulum membrane; GO:0045449 regulation of transcription; GO:0055036 virion membrane
Acknowledgements: Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory: For research purposes only

Applications of WNV antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA		1/1000	1/10000
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Mouse anti Histidine Tag:Alk.Phos.	MCA1396A	E WB	0.1 mg	Log in	Get Quote
Mouse anti Histidine Tag:Biotin	MCA1396B	E WB	0.1 mg	Log in	Get Quote
Mouse anti c-Myc:Alk.Phos.	MCA2200A	C E P WB*	0.1 mg	Log in	Get Quote
Mouse anti c-Myc:Biotin	MCA2200B	C E P WB*	0.1 mg	Log in	Get Quote
Mouse anti c-Myc:HRP	MCA2200P	C E P WB*	0.1 mg	Log in	Get Quote
Mouse anti Penta Histidine Tag:HRP	MCA5995P	E WB	125 µl	Log in	Get Quote

Product Specific References

References for WNV antibody

Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
J Biol Chem. 283 (25): 17270-8.
Shiryaev, S.A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
Biochem J. 427 (3): 369-76.

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