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WNV antibody | AbD05320

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Human anti WNV NS2B-NS3

Product Type
Monoclonal Antibody
Clone
AbD05320
Isotype
HuCAL Fab bivalent
Specificity
WNV
Region
NS2B-NS3

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
HCA085
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SDS Safety Datasheet SDS
E WB 0.1 mg loader
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Human anti WNV NS2B-NS3 antibody, clone AbD05320 recognizes the NS2B-NS3 proteinase from West Nile virus (WNV). West Nile virus is a member of the family Flaviviridae which in humans is transmitted via a mosquito vector resulting in central nervous damage. Non structural protein 3 (NS3) is a multifunctional protein, which is implicated in polyprotein processing and RNA replication. NS3 requires the presence of non-structural protein 2B (NS2B) cofactor to exhibit its proteolytic activity.

Target Species
Viral
Product Form
A bivalent human recombinant Fab (kappa light chain) selected from the HuCAL GOLD phage display library, expressed in E. coli.This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied liquid.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Immunogen
NS2B-NS3 proteinase from WNV expressed in E.coli
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000 1/10000
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.

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References for WNV antibody

  1. Chernov, A.V. et al. (2008) The two-component NS2B-NS3 proteinase represses DNA unwinding activity of the West Nile virus NS3 helicase.
    J Biol Chem. 283 (25): 17270-8.
  2. Shiryaev, S.A. et al. (2010) Isolation and characterization of selective and potent human Fab inhibitors directed to the active-site region of the two-component NS2B-NS3 proteinase of West Nile virus.
    Biochem J. 427 (3): 369-76.

RRID
AB_1605523
UniProt
P06935
Entrez Gene
WNVgp1
GO Terms
GO:0005515 protein binding
GO:0003724 RNA helicase activity
GO:0003725 double-stranded RNA binding
GO:0004252 serine-type endopeptidase activity
GO:0003968 RNA-directed RNA polymerase activity
GO:0016021 integral to membrane
GO:0004482 mRNA (guanine-N7-)-methyltransferase activity
GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity
GO:0005198 structural molecule activity
GO:0046872 metal ion binding
GO:0005524 ATP binding
GO:0006410 transcription, RNA-dependent
GO:0019079 viral genome replication
GO:0019028 viral capsid
GO:0019031 viral envelope
GO:0030683 evasion by virus of host immune response
GO:0042025 host cell nucleus
GO:0044167 host cell endoplasmic reticulum membrane
GO:0045449 regulation of transcription
GO:0055036 virion membrane
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.
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