V5-Tag antibody | SV5-Pk5
Mouse anti V5-tag antibody, clone SV5-Pk5 (MCA2895) used to identify V5 labeled proteins by western blotting.
Image caption:
PTEN is an ubiquitination target of Nedd4 and PTEN ubiquitination is decreased by Ndfip1 siRNA in HEK293T cells.
From: Hsu W-L, Ma Y-L, Chen Y-C, Liu Y-C, Cheng K-M, Lee EHY (2023)
Identification of Ndfip1 as a novel negative regulator for spatial memory formation associated with increased ubiquitination of Beclin 1 and PTEN.
PLoS ONE 18(4): e0283908.
doi: 10.1371/journal.pone.0283908
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti V5-Tag:FITC
- Product Type
- Monoclonal Antibody
- Clone
- SV5-Pk5
- Isotype
- IgG1
- Specificity
- V5-Tag
Antibody Quality and Performance Guarantee
| Mouse anti V5-Tag antibody, clone SV5-Pk5 recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. Mouse anti V5-Tag antibody, clone SV5-Pk5 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr. (The 9 amino acid epitope is underlined). |
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- A recombinant peptide corresponding to the PK tag fused to a class II - restricted T helper cell epitope.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the SP2/0 Ag14 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is manufactued under an exclusive license from the University of St. Andrews, UK.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/5 |
| Immunofluorescence | |
||
| Western Blotting | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for V5-Tag antibody
-
Dunn, C. et al. (1999) Fine mapping of the binding sites of monoclonal antibodies raised against the Pk tag.
J Immunol Methods. 224 (1-2): 141-50. -
Hsu, W.L. et al. (2023) Identification of Ndfip1 as a novel negative regulator for spatial memory formation associated with increased ubiquitination of Beclin 1 and PTEN.
PLoS One. 18 (4): e0283908.
- Synonyms
- PK-TAG
- RRID
- AB_1658048
MCA2895F
150617
152426
157299
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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