Poliovirus 1 antibody | 583-G8-G2-A4 (9D7/9)

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Mouse anti Poliovirus 1

Product Type
Monoclonal Antibody
Clone
583-G8-G2-A4 (9D7/9)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
7510-0000 IF 0.1 ml
Mouse anti Poliovirus 1 antibody, clone 583-G8-G2-A4 recognizes Poliovirus type 1, the causitive agent of most paralytic poliomyelitis cases and responsible for the majority of polio outbreaks during the first half of the 20th century.

Product Details

Target Species
Viral
Product Form
Ascites - liquid
Preservative Stabilisers
None present
Immunogen
Native poliovirus type 1.
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the Mouse Sp2/0 myeloma.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Poliovirus 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/100 1/400
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Product Specific References

Source Reference

  1. GaryHe, J.r. et al. (1997) Comparison of a monoclonal antibody-based IgM capture ELISA with a neutralization assay for assessing response to trivalent oral poliovirus vaccine.
    J Infect Dis. 175 Suppl 1: S264-7.

Further Reading

  1. Levintow, L. & DarnellJe, J.r. (1960) A simplified procedure for purification of large amounts of poliovirus: characterization and amino acid analysis of Type 1 poliovirus.
    J Biol Chem. 235: 70-3.