Infectious Salmon Anemia Virus (Isav) antibody | 4A11

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Mouse anti Infectious Salmon Anemia Virus (Isav)

Product Type
Monoclonal Antibody
Clone
4A11
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5977 C E IF 0.5 ml
Mouse anti Infectious Salmon Anemia Virus, clone 4A11, specifically recognizes the Infectious Salmon Anemia Virus, an orthomyxovirus being the sole member of the Isavirus genus. ISAV is the causative agent for Infectious Salmon Anemia (ISA), a notifiable disease affecting Atlantic salmon and resulting in high mortality (Weli, S.C., et al. 2013). ISAV is similar in structure to other orthomyxoviruses, with the exception of the hemagglutinin (HA) protein that would appear to be significantly different in size than for other influenza type viruses (Rimstad, E., et al. 2001)

ISAV was first identified in farmed salmon in Norway in 1984, spread by wild marine fish species, it has since been detected in farmed Atlantic salmon stocks throughout the North Atlantic, North West America and more recently in Chile, where it has also been detected in farmed Coho salmon (Godoy, M.G., et al. 2008). Due to the high mortality of infected individuals, ISAV has a significant ecconomic impact and infections are of serious concern to the aquaculture industry.

While the route of entry into the host, is as yet, poorly understood, evidence indicates that the virus enters through epithelial tissue in the gills (Weli, S.C., et al. 2013), this is similar to mammalian orthomyxoviruses which target respiratory tissues.

Clone 4A11can be used to identify ISAV in the tissues of infected fish and may be of use for research into methods for diagnosis and control of ISAV infections in the aquaculture industry.

Product Details

Target Species
Viral
Product Form
Tissue Culture Supernatant - liquid (concentrated)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
ISAV isolate
Approx. Protein Concentrations
IgG concentration 0.5 - 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Mouse SP2/0-Ag-14 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q8V3T7 Related reagents
Q8V3U0 Related reagents
Entrez Gene
NP Related reagents
HA Related reagents
GO Terms
GO:0003676 nucleic acid binding
GO:0019012 virion
GO:0042025 host cell nucleus
GO:0016021 integral to membrane
GO:0016787 hydrolase activity
GO:0033644 host cell membrane
Regulatory
For research purposes only

Applications of Infectious Salmon Anemia Virus (Isav) antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit anti Salmonid Ig AHP761 50 µl E IF WB
Mouse anti Salmonid Ig MCA2182 0.5 ml E F IF WB
Mouse anti Rainbow Trout Ig MCA5976 0.5 ml C E IF WB

Product Specific References

Further Reading

  1. Kent, M.L. (2000) Marine netpen farming leads to infections with some unusual parasites.
    Int J Parasitol. 30: 321-6.
  2. Aamelfot, M., et al. (2013) Characterisation of a monoclonal antibody detecting Atlantic salmon endothelial and red blood cells, and its association with the infectious salmon anaemia virus cell receptor.
    J Anat. 222(5): 547-57.
  3. Lyngstad, T.M., et al. (2012) Low virulent infectious salmon anaemia virus (ISAV-HPR0) is prevalent and geographically structured in Norwegian salmon farming.
    Dis Aquat Organ. 101: 197-206.
  4. Weli, S.C., et al. (2013) Infectious salmon anaemia virus infection of Atlantic salmon gill epithelial cells.
    Virol J. 10: 5
  5. Falk, K., et al. (1998) Characterization and applications of a monoclonal antibody against infectious salmon anaemia virus.
    Dis. Aquat. Org. 34: 77-85.
  6. Rimstad, E. et al. (2001) Characterization of the infectious salmon anemia virus genomic segment that encodes the putative hemagglutinin.
    J Virol. 75 (11): 5352-6.
  7. Godoy, M.G., et al. (2008) First detection, isolation and molecular characterization of infectious salmon anaemia virus associated with clinical disease in farmed Atlantic salmon (Salmo salar) in Chile.
    BMC Vet Res. 4: 28.