gp41 antibody | AbD08066

Human anti gp41:Preservative Free

Product Type
Monoclonal Antibody
Clone
AbD08066
Isotype
HuCAL Fab monovalent
Specificity
gp41

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HCA239Z
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E FN * 0.1 mg loader
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Human Anti-gp41 Antibody, clone AbD08066 recognizes an epitope derived from the ectodomain of the Human Immunodeficiency Virus (HIV) type 1 envelope glycoprotein 41 (gp41).

This antibody was selected from the Human Combinatorial Antibody Library (HuCAL®) by panning on an HIV gp41-derived chimeric construct, NCCG-gp41, as described in Louis et al. 2001.

The fusion of HIV-1 with the membrane of the host cell is the first step in the process of viral infection and replication and involves conformational changes in the gp120 and gp41 viral envelope proteins. It is now known that the HIV gp41 contains a highly conserved N-heptad repeat (N-HR) region which is conserved in many HIV-1 strains and as such it is hypothesized that this maybe a target for developing a vaccine (Gustchina et al. 2010).

Clone AbD8066 is a unique antibody that has been shown to be broadly neutralizing across a wide panel of B and C type HIV-1 viruses. The crystal structure of the N-HR mimetic 5-Helix with this antibody has been reported (Gustchina et al. 2010, Protein Data Bank ID 3MA9).

Target Species
Viral
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
None present.
Immunogen
Recombinant protein corresponding to a conserved internal trimeric coiled-coil of the N-heptad repeat (N-HR) of HIV-1 gp41.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.

Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Functional Assays 1
  1. 1This antibody demonstrates neutralizing properties
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
This product is suitable for use in indirect ELISA applications.

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Description HuCAL Fab-MH Negative Control

References for gp41 antibody

  1. Gustchina, E. et al. (2010) Structural basis of HIV-1 neutralization by affinity matured Fabs directed against the internal trimeric coiled-coil of gp41.
    PLoS Pathog. 6: e1001182.
  2. Gustchina, E. et al. (2009) Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
    Virology. 393: 112-9.
  3. Gustchina, E et al. (2013) Complexes of Neutralizing and Non-Neutralizing Affinity Matured Fabs with a Mimetic of the Internal Trimeric Coiled-Coil of HIV-1 gp41.
    PLoS One. 8: e78187

Functional Assays

Protein Purification

Western Blotting

Synonyms
HIV1 ENV
UniProt
Q76270
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
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