gp41 antibody | AbD08066

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Human anti gp41:Preservative Free

Product Type
Monoclonal Antibody
HuCAL Fab monovalent
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Human Anti-gp41 Antibody, clone AbD08066 recognizes an epitope derived from the ectodomain of the Human Immunodeficiency Virus (HIV) type 1 envelope glycoprotein 41 (gp41).

This antibody was selected from the Human Combinatorial Antibody Library (HuCAL®) by panning on an HIV gp41-derived chimeric construct, NCCG-gp41, as described in Louis et al. 2001.

The fusion of HIV-1 with the membrane of the host cell is the first step in the process of viral infection and replication and involves conformational changes in the gp120 and gp41 viral envelope proteins. It is now known that the HIV gp41 contains a highly conserved N-heptad repeat (N-HR) region which is conserved in many HIV-1 strains and as such it is hypothesized that this maybe a target for developing a vaccine (Gustchina et al. 2010).

Clone AbD8066 is a unique antibody that has been shown to be broadly neutralizing across a wide panel of B and C type HIV-1 viruses. The crystal structure of the N-HR mimetic 5-Helix with this antibody has been reported (Gustchina et al. 2010, Protein Data Bank ID 3MA9).

Product Details

Target Species
Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Recombinant protein corresponding to a conserved internal trimeric coiled-coil of the N-heptad repeat (N-HR) of HIV-1 gp41.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml

Storage Information

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch

More Information

This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
For research purposes only

Applications of gp41 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays 1
  1. 1This antibody demonstrates neutralizing properties
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
This product is suitable for use in indirect ELISA applications.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
HuCAL Fab-MH Negative Control HCA051 E 0.1 mg loader

Application Based External Images

Functional Assays

Protein Purification

Western Blotting

Product Specific References

References for gp41 antibody

  1. Gustchina, E. et al. (2010) Structural basis of HIV-1 neutralization by affinity matured Fabs directed against the internal trimeric coiled-coil of gp41.
    PLoS Pathog. 6: e1001182.
  2. Gustchina, E. et al. (2009) Affinity maturation by targeted diversification of the CDR-H2 loop of a monoclonal Fab derived from a synthetic naïve human antibody library and directed against the internal trimeric coiled-coil of gp41 yields a set of Fabs with improved HIV-1 neutralization potency and breadth.
    Virology. 393: 112-9.
  3. Gustchina, E et al. (2013) Complexes of Neutralizing and Non-Neutralizing Affinity Matured Fabs with a Mimetic of the Internal Trimeric Coiled-Coil of HIV-1 gp41.
    PLoS One. 8: e78187

Fluorescent Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
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  • Select combined or multi-laser view to visualize the spectra