Foot-and-Mouth Disease Virus Non-Structural Protein 2a antibody | 1G2

Mouse anti Foot-and-Mouth Disease Virus Non-Structural Protein 2a

Product Type
Monoclonal Antibody
Clone
1G2
Isotype
IgG2a
Specificity
Foot-and-Mouth Disease Virus Non-Structural Protein 2a

Product Code Applications Pack Size List Price Your Price Qty
MCA2854
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.2 mg loader
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loader
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Mouse anti Foot-and-Mouth Disease Virus Non-Structural Protein 2a antibody, clone 1G2 recognizes the 2a non-structural protein of Foot-and-mouth disease virus (FMDV). Foot-and-mouth disease virus is a nonenveloped icosahedral Aphthovirus that contains single-stranded RNA. Foot-and-mouth disease is a highly contagious and sometimes fatal disease of cattle, pigs and other cloven-hoofed animals. The disease is characterised by high fever and blisters inside the mouth and on the feet. Although it doesn’t have a high mortality rate, the disease has debilitating effects, including weight loss and decrease in milk production, resulting in detrimental economic ramifications. The virus is spread through direct contact.

The non-structural proteins are necessary for viral replication and as such mutations in this area are probably less well tolerated than in the rest of the genome. Non-structural proteins have been targeted as possible diagnostic targets to distinguish between infected and vaccinated animals..

Note: clone 1G2 was previously described in error to recognize Non-Structural Protein 3a.

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Recombinant FMDV non structural protein 2a.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for Foot-and-Mouth Disease Virus Non-Structural Protein 2a antibody

  1. Grubman, M. J. and Baxt, B. (2004) Foot-and-mouth disease.
    Clin Microbiol Rev. 17: 465-493.

Synonyms
FMDV
RRID
AB_1125315
UniProt
P49303
GO Terms
GO:0004197 cysteine-type endopeptidase activity
GO:0003723 RNA binding
GO:0003724 RNA helicase activity
GO:0016020 membrane
GO:0003968 RNA-directed RNA polymerase activity
GO:0005198 structural molecule activity
GO:0005524 ATP binding
GO:0006410 transcription, RNA-dependent
GO:0006508 proteolysis
GO:0019079 viral genome replication
GO:0018144 RNA-protein covalent cross-linking
GO:0019030 icosahedral viral capsid
GO:0019082 viral protein processing
GO:0044162 host cell cytoplasmic vesicle membrane
GO:0044419 interspecies interaction between organisms
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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