Feline Immunodeficiency Virus p24 antibody | vpg50

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Mouse anti Feline Immunodeficiency Virus p24

Product Type
Monoclonal Antibody
Clone
vpg50
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1353 F IF IP WB 2 ml
Mouse anti Feline Immunodeficiency Virus p24 antibody, clone vpg50 recognizes feline immunodeficiency virus (FIV) p24, the ~24 kDa capsid protein which forms the conical core of the virus and encapsulates the genomic RNA-nucleocapsid complex.

Mouse anti Feline Immunodeficiency Virus p24 antibody, clone vpg50 has been used successfully for the detection of p24 in FIV infected dendritic cells by immunocytochemistry and detection of both recombinant p24 and p24 from virally infected cell cultures by western blotting (Reggeti and Bienzle 2008).

Product Details

Target Species
Viral
Product Form
Tissue Culture Supernatant -liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4
Preservative Stabilisers
0.1%Sodium Azide
Immunogen
Recombinant p24.
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS0 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC for one month or at -20oC for longer.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

Regulatory
For research purposes only

Applications of Feline Immunodeficiency Virus p24 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Western Blotting 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for Feline Immunodeficiency Virus p24 antibody

  1. Dowling, R.J. & Bienzle, D. (2005) Gene-expression changes induced by Feline immunodeficiency virus infection differ in epithelial cells and lymphocytes.
    J Gen Virol. 86 (Pt 8): 2239-48.
  2. Reggeti, F. and Bienzle, D. (2008) Alloimmunity does not protect from challenge with the feline immunodeficiency virus.
    Vet Immunol Immunopathol. 124: 152-62.
  3. Rogers, A.B. and Hoover, E.A. (2002) Fetal Feline Immunodeficiency Virus Is Prevalent and Occult
    J Infect Dis. 186: 895-904.
  4. Dietrich, I. et al. (2011) Feline tetherin efficiently restricts release of feline immunodeficiency virus but not spreading of infection.
    J Virol. 85: 5840-52.
  5. Rolim, V.M. et al. (2016) Myocarditis caused by Feline Immunodeficiency Virus in Five Cats with Hypertrophic Cardiomyopathy.
    J Comp Pathol. 154 (1): 3-8.