Astrovirus antibody | 8E7

Mouse anti Astrovirus

Product Type
Monoclonal Antibody
Clone
8E7
Isotype
IgG1
Specificity
Astrovirus

Product Code Applications Pack Size List Price Your Price Qty
MCA2716
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E IP 1 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Astrovirus antibody, clone 8E7 recognises a genus specific epitope within the viral capsid protein of all human astrovirus serotypes. Mouse anti Astrovirus antibody, clone 8E7 does not cross-react with rotavirus (SA11), SRSV, adenovirus type 2, 40 and 41, norwalk virus 8F11, Hawaii virus, coxsackievirus types A9 and B2, calcivirus and snow mountain virus.

Astrovirus is a non-enveloped single-stranded RNA virus of the Astroviridae family, and a common causative agent of gastroenteritis in the young and adults (Oishi et al. 1994).

Target Species
Viral
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Astrovirus type 2 expressed in LLC MK2 cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed) STAR117D550 F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed) STAR117D650 F IF 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
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Description Goat anti Mouse IgG (Fc):FITC
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
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Description Goat anti Mouse IgG (Fc):HRP
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

References for Astrovirus antibody

  1. Geigenmüller, U. et al. (2002) Studies on intracellular processing of the capsid protein of human astrovirus serotype 1 in infected cells.
    J Gen Virol. 83 (Pt 7): 1691-5.
  2. Geigenmüller U et al. (1997) Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.
    J Virol. 71 (2): 1713-7.
  3. Lewis, T.L. et al. (1994) Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein.
    J Virol. 68 (1): 77-83.
  4. Noel, J.S. et al. (1995) Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing.
    J Clin Microbiol. 33 (4): 797-801.
  5. Herrmann JE et al. (1988) Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies.
    J Infect Dis. 158 (1): 182-5.
  6. Oishi, I. et al. (1994) A large outbreak of acute gastroenteritis associated with astrovirus among students and teachers in Osaka, Japan.
    J Infect Dis. 170 (2): 439-43.
  7. Guix, S. et al. (2004) Apoptosis in astrovirus-infected CaCo-2 cells.
    Virology. 319 (2): 249-61.
  8. Kriston, S. et al. (1996) Seroprevalence of astrovirus types 1 and 6 in London, determined using recombinant virus antigen.
    Epidemiol Infect. 117 (1): 159-64.
  9. View The Latest Product References
  10. Guix, S. et al. (2004) C-terminal nsP1a protein of human astrovirus colocalizes with the endoplasmic reticulum and viral RNA.
    J Virol. 78 (24): 13627-36.
  11. Sebire, N.J. et al. (2004) Pathology of astrovirus associated diarrhoea in a paediatric bone marrow transplant recipient.
    J Clin Pathol. 57 (9): 1001-3.
  12. Caballero, S. et al. (2004) Structural requirements of astrovirus virus-like particles assembled in insect cells.
    J Virol. 78 (23): 13285-92.

Further Reading

  1. Arias, C.F. & DuBois, R.M. (2017) The Astrovirus Capsid: A Review.
    Viruses. 9 (1): .

Immunohistology - Paraffin

RRID
AB_915148
UniProt
Q82446
GO Terms
GO:0019028 viral capsid
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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