Astrovirus antibody | 8E7
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Mouse anti Astrovirus antibody, clone 8E7 recognises a genus specific epitope within the viral capsid protein of all human astrovirus serotypes. Mouse anti Astrovirus antibody, clone 8E7 does not cross-react with rotavirus (SA11), SRSV, adenovirus type 2, 40 and 41, norwalk virus 8F11, Hawaii virus, coxsackievirus types A9 and B2, calcivirus and snow mountain virus.
Astrovirus is a non-enveloped single-stranded RNA virus of the Astroviridae family, and a common causative agent of gastroenteritis in the young and adults (Oishi et al. 1994).
Astrovirus is a non-enveloped single-stranded RNA virus of the Astroviridae family, and a common causative agent of gastroenteritis in the young and adults (Oishi et al. 1994).
Product Details
- Target Species
- Viral
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Astrovirus type 2 expressed in LLC MK2 cells.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q82446
- GO Terms
- GO:0019028 viral capsid
- Regulatory
- For research purposes only
Applications of Astrovirus antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunoprecipitation |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for Astrovirus antibody
-
Geigenmüller, U. et al. (2002) Studies on intracellular processing of the capsid protein of human astrovirus serotype 1 in infected cells.
J Gen Virol. 83 (Pt 7): 1691-5. -
Geigenmüller U et al. (1997) Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.
J Virol. 71 (2): 1713-7. -
Lewis, T.L. et al. (1994) Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein.
J Virol. 68 (1): 77-83. -
Noel, J.S. et al. (1995) Typing of human astroviruses from clinical isolates by enzyme immunoassay and nucleotide sequencing.
J Clin Microbiol. 33 (4): 797-801. -
Herrmann JE et al. (1988) Antigenic characterization of cell-cultivated astrovirus serotypes and development of astrovirus-specific monoclonal antibodies.
J Infect Dis. 158 (1): 182-5. -
Oishi, I. et al. (1994) A large outbreak of acute gastroenteritis associated with astrovirus among students and teachers in Osaka, Japan.
J Infect Dis. 170 (2): 439-43. -
Guix, S. et al. (2004) Apoptosis in astrovirus-infected CaCo-2 cells.
Virology. 319 (2): 249-61. -
Kriston, S. et al. (1996) Seroprevalence of astrovirus types 1 and 6 in London, determined using recombinant virus antigen.
Epidemiol Infect. 117 (1): 159-64. -
Guix, S. et al. (2004) C-terminal nsP1a protein of human astrovirus colocalizes with the endoplasmic reticulum and viral RNA.
J Virol. 78 (24): 13627-36. -
Sebire, N.J. et al. (2004) Pathology of astrovirus associated diarrhoea in a paediatric bone marrow transplant recipient.
J Clin Pathol. 57 (9): 1001-3. -
Caballero, S. et al. (2004) Structural requirements of astrovirus virus-like particles assembled in insect cells.
J Virol. 78 (23): 13285-92.
Further Reading
-
Arias, C.F. & DuBois, R.M. (2017) The Astrovirus Capsid: A Review.
Viruses. 9 (1): .
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